Anti-PUS10 抗体 (ab185078)
Key features and details
- Rabbit polyclonal to PUS10
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PUS10 antibody
PUS10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PUS10 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human PUS10 aa 250-400 (internal sequence).
Database link: Q3MIT2 -
ポジティブ･コントロール
- WB: HepG2 cell line. IHC-P: Human stomach, testis, tonsil, cerebral cortex and thyroid gland tissue. ICC/IF: A431 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185078の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. -
配列類似性
Belongs to the pseudouridine synthase Pus10 family. -
翻訳後修飾
Proteolytically cleaved during TRAIL-induced cell death. Cleaved, in vitro, either by caspase-3 or caspase-8.
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 150962 Human
- Entrez Gene: 74467 Mouse
- Entrez Gene: 305583 Rat
- Omim: 612787 Human
- SwissProt: Q3MIT2 Human
- SwissProt: Q9D3U0 Mouse
- Unigene: 368348 Human
- Unigene: 29536 Mouse
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別名
- CCDC139 antibody
- Coiled coil domain containing 139 antibody
- Coiled coil domain containing protein 139 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody (ab185078)
Immunohistochemistry analysis of human cerebral cortex tissue labeling PUS10 with ab185078 at a 1/50 dilution.
Moderate nuclear and cytoplasmic positivity in neuronal cells.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody (ab185078)Immunohistochemistry analysis of human tonsil tissue labeling PUS10 with ab185078 at a 1/50 dilution.
Moderate nuclear and cytoplasmic positivity in squamous epithelial cells.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody (ab185078)Immunohistochemistry analysis of human testis tissue labeling PUS10 with ab185078 at a 1/50 dilution.
Moderate nuclear and cytoplasmic positivity in seminiferous ducts.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody (ab185078)Immunohistochemistry analysis of human thyroid gland tissue labeling PUS10 with ab185078 at a 1/50 dilution.
Moderate nuclear positivity in glandular cells.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-PUS10 antibody (ab185078)Anti-PUS10 antibody (ab185078) at 0.4 µg/ml + Human cell line HepG2
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody (ab185078)Immunohistochemistry analysis of human stomach tissue labeling PUS10 with ab185078 at a 1/50 dilution.
Moderate nuclear and cytoplasmic positivity in glandular cells.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-PUS10 antibody (ab185078)Immunocytochemistry analysis of A431 (human epidermoid carcinoma cell line) whole cells labeling PUS10 nucleoplasm, nuclear bodies and mitochondria with ab185078 at 2 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185078 は論文での使用が確認できていません。