AB228743

Anti-Pumilio 2 抗体 - N-terminal

Anti-Pumilio 2 antibody - N-terminal

JP Deleterious:医薬用外劇物

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(1 Publication)

Rabbit Polyclonal Pumilio 2 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PUM2.

別名を表示する

KIAA0235, PUMH2, PUM2, Pumilio homolog 2, Pumilio-2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 2 antibody - N-terminal (AB228743)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Paraffin-embedded mouse kidney tissue stained for Pumilio 2 with ab228743 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

5% SDS-PAGE

All lanes:

Western blot - Anti-Pumilio 2 antibody - N-terminal (ab228743) at 1/5000 dilution

All lanes:

Human ESC whole cell lysate at 20 µg

Predicted band size: 114 kDa

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Supplier Data

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Pumilio 2 antibody - N-terminal (ab228743) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Predicted band size: 114 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human PUM2. The exact immunogen used to generate this antibody is proprietary information.

Q8TB72

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Pumilio 2 also known as PUM2 is a member of the PUF family of RNA-binding proteins. It has an approximate mass of 120 kDa. PUM2 is expressed in a variety of tissues including the brain testis and ovary. The protein primarily interacts with the 3'-untranslated regions (3' UTRs) of target mRNAs influencing their stability and translation. PUM2 acts by binding to specific sequences which mediates post-transcriptional regulation of gene expression.

Biological function summary

The regulation of mRNA stability and translation by PUM2 contributes to critical cellular processes such as development and differentiation. PUM2's involvement is observed within the context of RNA-protein complexes where it functions as an important regulator of mRNA fate. Its activity allows cells to tailor protein production according to specific needs such as maintaining stem cell pluripotency or guiding germ cell development.

Pathways

PUM2 participates in the regulation of mRNA translation and degradation aligning with pathways like the RNA processing and cell cycle pathways. In these pathways PUM2 collaborates with elements like the DCP1-DCP2 decapping complex to influence mRNA longevity and turnover. It also interacts with proteins such as NANOS enabling fine-tuned control over cell cycle progression and differentiation.

Altered expression of PUM2 has ties to neurological conditions like intellectual disability and infertility-related disorders. Mutations or misregulation in PUM2 expression can disrupt normal neural function with impacts linked to proteins like FMRP which is associated with fragile X syndrome. In reproductive diseases PUM2 regulation of specific mRNAs influences germline stem cell maintenance connecting its role to fertility issues.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed : 21397187). Mediates post-transcriptional repression of transcripts via different mechanisms : acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed : 22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed : 18776931, PubMed : 22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed : 22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed : 25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed : 26724866). May regulate DCUN1D3 mRNA levels (PubMed : 25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed : 28431233).

See full target information PUM2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1234577 PubMed37854606

2023

Single-cell profiling reveals immune disturbances landscape and HLA-F-mediated immune tolerance at the maternal-fetal interface in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Luo,Fulin Liu,Yingzhe Guo,Wenming Xu,Yilin Li,Jun Yi,Thierry Fournier,Séverine Degrelle,Hedia Zitouni,Isabelle Hernandez,Xinghui Liu,Yu Huang,Jun Yue

View all publications

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pumilio 2 抗体 - N-terminal

Anti-Pumilio 2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise