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Anti-PU.1/Spi1 抗体 [2G1] (ab88082)

Submit a review Submit a question References (9)
Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
  • Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
  • ELISA - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
  • Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
  • Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
  • Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (ab88082)

Key features and details

  • Mouse monoclonal [2G1] to PU.1/Spi1
  • Suitable for: WB, ELISA, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-PU.1/Spi1 antibody [2G1]
    PU.1/Spi1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2G1] to PU.1/Spi1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ELISA, ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Human, Recombinant fragment

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa cells; Raw 264.7 cell lysate.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab88082の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

  • 配列類似性

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    画像

    • Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      Anti-PU.1/Spi1 antibody [2G1] (ab88082) at 5 µg/ml + tagged human recombinant protein fragment (the immunogen) at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size: 31 kDa
      Observed band size: 39 kDa why is the actual band size different from the predicted?



      Note: Molecular weight of the proprietary tag alone is 26 kDa.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)

      ab88082, at 10 µg/ml, staining PU.1/Spi1 in HeLa cells by Immunofluorescence.

      This image was generated using the ascites version of the product. 

    • ELISA - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      ELISA - Anti-PU.1/Spi1 antibody [2G1] (ab88082)

      Detection limit for recombinant (human) tagged ab88082 is approximately 0.1ng/ml as a capture antibody.

      This image was generated using the ascites version of the product. 

    • Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      Anti-PU.1/Spi1 antibody [2G1] (ab88082) at 5 µg/ml + Raw 264.7 cell lysate (Mouse) at 25 µg

      Secondary
      Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa



      This image was generated using the ascites version of the product. 

    • Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)Image courtesy of Sellgren et al. Mol Psychiatry. 2017 Feb; 22(2): 170–177. Fig S3: Doi: 10.1038/mp.2016.220

      Ab88082 staining PU.1/Spi1 in human induced microglia by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde for 15 minutes. Samples were incubated with primary antibody (1:200) for 2 hours at room temperature. Hoechst nuclear stain (blue) was used at 1:5000 dilution. 

      This image was generated using the ascites version of the product. 

    • Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
      Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (ab88082)

      Overlay histogram showing K562 cells stained with ab88082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab88082, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This image was generated using the ascites version of the product. 

    参考文献 (9)

    ab88082 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab88082 は 9 報の論文で使用されています。

    • Chen Y  et al. miRNA-124 Prevents Rat Diabetic Retinopathy by Inhibiting the Microglial Inflammatory Response. Int J Mol Sci 24:N/A (2023). PubMed: 36768614
    • Bašová P  et al. Combined Approach to Leukemic Differentiation Using Transcription Factor PU.1-Enhancing Agents. Int J Mol Sci 23:N/A (2022). PubMed: 35743167
    • Li Q  et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun 13:7677 (2022). PubMed: 36509766
    • Zha Z  et al. Bu Shen Yi Sui Capsule Alleviates Neuroinflammation and Demyelination by Promoting Microglia toward M2 Polarization, Which Correlates with Changes in miR-124 and miR-155 in Experimental Autoimmune Encephalomyelitis. Oxid Med Cell Longev 2021:5521503 (2021). PubMed: 33815654
    • Banerjee A  et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci 15:629279 (2021). PubMed: 33897370
    • Chen Y  et al. MicroRNA-124 Alleviates Retinal Vasoregression via Regulating Microglial Polarization. Int J Mol Sci 22:N/A (2021). PubMed: 34681723
    • You MJ  et al. A new method for obtaining bankable and expandable adult-like microglia in mice. J Neuroinflammation 18:294 (2021). PubMed: 34920745
    • Sellgren CM  et al. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry 22:170-177 (2017). ICC/IF ; Human . PubMed: 27956744
    • Zhou J  et al. PU.1 is essential for MLL leukemia partially via crosstalk with the MEIS/HOX pathway. Leukemia 28:1436-48 (2014). PubMed: 24445817

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