Anti-PTPRR/PCPTP1 抗体 [EPR13188] (ab180134)
![Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)](https://www.abcam.co.jp/ps/products/180/ab180134/Images/ab180134-210058-ab180134-wb.jpg "Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13188] to PTPRR/PCPTP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PTPRR/PCPTP1 antibody [EPR13188]
PTPRR/PCPTP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13188] to PTPRR/PCPTP1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PCPTP1/PTPRQ aa 1-100. The exact sequence is proprietary.
Database link: Q15256 -
ポジティブ･コントロール
- Human cerebellum, mouse and rat brain lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13188 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180134の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 74 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 74 kDa. |
ターゲット情報
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機能
Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. -
組織特異性
Expressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
細胞内局在
Cell membrane and Cytoplasm > perinuclear region. Locates to the perinuclear areas within the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5801 Human
- Entrez Gene: 19279 Mouse
- Entrez Gene: 94202 Rat
- Omim: 602853 Human
- SwissProt: Q15256 Human
- SwissProt: Q62132 Mouse
- SwissProt: O08617 Rat
- Unigene: 506076 Human
see all -
別名
- Ch-1PTPase antibody
- Ch1 PTPase antibody
- DKFZp781C1038 antibody
see all
画像
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Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)All lanes : Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134) at 1/5000 dilution
Lane 1 : Human cerebellum lysate at 20 µg
Lane 2 : Mouse brain lysate at 20 µg
Lane 3 : Rat brain lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDa -
![Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)](https://www.abcam.co.jp/ps/products/180/ab180134/Images/ab180134-487225-anti-pcptp1-ptprq-antibody-epr13188-western-blot-human.jpg)
Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)All lanes : Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
Lane 3 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate
Lane 4 : Human cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM /TBSTHEK-293, HCT116 and SK-OV-3 are low expressors (PMID: 25592066, PMID: 20015382, PMID: 31653698).
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![Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)](https://www.abcam.co.jp/ps/products/180/ab180134/Images/ab180134-2-benefits-of-recombinant-antibodies.png)
Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab180134 は 1 報の論文で使用されています。
- Chang Y et al. Parvimonas micra activates the Ras/ERK/c-Fos pathway by upregulating miR-218-5p to promote colorectal cancer progression. J Exp Clin Cancer Res 42:13 (2023). PubMed: 36627634