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Anti-PTPRK 抗体 (ab13225)

Submit a review Q&A (2)References (2)
Western blot - Anti-PTPRK antibody (ab13225)

    Key features and details

    • Rabbit polyclonal to PTPRK
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-PTPRK antibody
      PTPRK 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to PTPRK

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse

    • 免疫原

      Synthetic peptide corresponding to Human PTPRK aa 900-1000 (internal sequence) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab13232)

    • ポジティブ･コントロール

      • This antibody gave a positive result in PTP beta (PTPRB) and PTPRK recombinant protein domains

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab13225の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).
    特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).

    ターゲット情報

    • 機能

      Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTP-kappa.

    • 組織特異性

      High levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma.

    • 配列類似性

      Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
      Contains 4 fibronectin type-III domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 MAM domain.
      Contains 2 tyrosine-protein phosphatase domains.

    • 翻訳後修飾

      This protein undergoes proteolytic processing.

    • 細胞内局在

      Cell junction > adherens junction. Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • dJ480J14.2.1 antibody
        • DKFZp686C2268 antibody
        • DKFZp779N1045 antibody
        • OTTHUMP00000017180 antibody
        • OTTHUMP00000017181 antibody
        • OTTHUMP00000017182 antibody
        • OTTHUMP00000017187 antibody
        • OTTHUMP00000040306 antibody
        • Protein tyrosine phosphatase kappa antibody
        • Protein tyrosine phosphatase kappa precursor antibody
        • Protein tyrosine phosphatase receptor type K antibody
        • Protein tyrosine phosphatase receptor type kappa antibody
        • Protein-tyrosine phosphatase kappa antibody
        • PTPK antibody
        • PTPkappa antibody
        • Ptprk antibody
        • PTPRK_HUMAN antibody
        • R PTP kappa antibody
        • R-PTP-kappa antibody
        • Receptor type tyrosine protein phosphatase kappa antibody
        • Receptor-type tyrosine-protein phosphatase kappa antibody
        see all

      画像

      • Western blot - Anti-PTPRK antibody (ab13225)
        Western blot - Anti-PTPRK antibody (ab13225)
        All lanes : Anti-PTPRK antibody (ab13225) at 1 µg/ml

        Lane 1 : 0.1µg GLEPP1 (PTPRO) protein
        Lane 2 : 0.1µg SAP 1 (PTPRH) protein
        Lane 3 : 0.1µg PTP gamma (PTPRG) protein
        Lane 4 : 0.1µg PTP beta (PTPRB) protein
        Lane 5 : 0.1µg DEP1 (PTPRJ) protein
        Lane 6 : 0.1µg PTP kappa (PTPRK) protein
        Lane 7 : 0.1µg PCPTP1 (PTPRR) protein
        Lane 8 : 0.1µg LAR (PTPRF) protein

        Performed under reducing conditions.

        Predicted band size: 50-70 kDa
        Observed band size: 65 kDa why is the actual band size different from the predicted?



        Recombinant PTP phosphatase domains (0.1µg of protein) were probed with ab13225 at 1/1000.  The antibody recognises the recombinant PTPRK fusion protein at ~65 kDa. Ladder sizes are in kilodaltons.

        Lane #         Recombinant Protein

        1                  GLEPP1 (PTP RO)
        2                  SAP 1 (PTP RH)
        3                  PTP gamma (PTP RG)
        4                  PTP beta (PTP B)
        5        &nbsp

      参考文献 (2)

      ab13225 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab13225 は 2 報の論文で使用されています。

      • Agarwal S  et al. Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma. PLoS One 8:e62852 (2013). WB ; Human . PubMed: 23696788
      • Flavell JR  et al. Down-regulation of the TGF-beta target gene, PTPRK, by the Epstein-Barr virus encoded EBNA1 contributes to the growth and survival of Hodgkin lymphoma cells. Blood 111:292-301 (2008). PubMed: 17720884

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      Customer contacted us to inquire about the WB image and expected band size when using this product.

      Read More
      Answer

      Thank you for contacting us.

      I have been informed that this product is tested using recombinant PTP phosphatase domains. The expected size for these recombinant peptides is around ˜65kDa. We have not tested this in endogenous systems however it is expected that PTPk targets will be around 160 kDa.


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Question

      Inquiry: I am looking for an antibody that will work on Human PTPRK in an immunoblot and with FACS as well. Can you tell me if ab13225 will do the job? Does it bind inter or extracellularly?

      Read More
      Answer

      Thank you for your enquiry and your interest.

      This is a polyclonal antibody so it binds to different epitopes. Thus for polyclonal antibodies epitope mapping is usually not carried out.

      I can confirm that ab13225 has only been tested on Western blot application. Currently, we do not have any information if ab13225 recognizes the native form of the protein or only the denatured form.

      I could offer you a testing discount if you wish to test it in Flow cytometry/FACS. I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: www.abcam.com/abtrial.

      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

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