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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PTP1B 抗体 [EP1841Y] (ab75856)

Submit a review Submit a question References (4)
Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856)
  • Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EP1841Y] (ab75856)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EP1841Y] (ab75856)
  • Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (ab75856)
  • Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856)
  • Anti-PTP1B antibody [EP1841Y] (ab75856)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1841Y] to PTP1B
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP

製品の概要

  • 製品名

    Anti-PTP1B antibody [EP1841Y]
    PTP1B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1841Y] to PTP1B

  • 由来種

    Rabbit

  • 特異性

    ab75856 detects both phosphorylated and nonphosphorylated PTP1B.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HepG2 or Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. Flow Cyt (intra): Jurkat cells. ICC/IF: HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP1841Y

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75856の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/500.
特記事項
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/500.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    May play an important role in CKII- and p60c-src-induced signal transduction cascades.

  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.

  • 翻訳後修飾

    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.

  • 細胞内局在

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    画像

    • Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856)
      Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PTPB1 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab75856 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab75856 was shown to specifically react with PTP1B when PTP1B knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab75856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EP1841Y] (ab75856)
      Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EP1841Y] (ab75856)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PTP1B with purified ab75856 at 1/500 dilution (5.3 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EP1841Y] (ab75856)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EP1841Y] (ab75856)

      ab75856, at 1/50 dilution, staining PTP1B in human tonsil, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (ab75856)
      Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (ab75856)

      Intracellular Flow Cytometry analysis of Jurkat cells labelling PTP1B with ab75856 at 1/2000 (red). Cells were fixed with 90% methanol. ab150077, Alexa Fluorr® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG (ab172730). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

       

       

    • Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856)
      Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856)
      All lanes : Anti-PTP1B antibody [EP1841Y] (ab75856) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa

    • Anti-PTP1B antibody [EP1841Y] (ab75856)
      Anti-PTP1B antibody [EP1841Y] (ab75856)

    参考文献 (4)

    ab75856 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab75856 は 4 報の論文で使用されています。

    • Xu X  et al. miR-125a-5p inhibits tumorigenesis in hepatocellular carcinoma. Aging (Albany NY) 11:7639-7662 (2019). PubMed: 31527306
    • Radmanesh F  et al. Optimization of miRNA delivery by using a polymeric conjugate based on deoxycholic acid-modified polyethylenimine. Int J Pharm 565:391-408 (2019). PubMed: 31085260
    • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
    • Weidmann MD  et al. Mena(INV) dysregulates cortactin phosphorylation to promote invadopodium maturation. Sci Rep 6:36142 (2016). PubMed: 27824079

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