AB75856

Anti-PTP1B 抗体 [EP1841Y]

Anti-PTP1B antibody [EP1841Y]

RabMAb
Recombinant
KO Validated
詳細を見る

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal PTP1B antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

別名を表示する

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

6 Images

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (AB75856)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (AB75856)

Intracellular Flow Cytometry analysis of Jurkat cells labelling PTP1B with ab75856 at 1/2000 (red). Cells were fixed with 90% methanol. ab150077, Alexa Fluorr^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG (ab172730). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EP1841Y] (AB75856)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EP1841Y] (AB75856)

ab75856, at 1/50 dilution, staining PTP1B in human tonsil, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EP1841Y] (AB75856)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EP1841Y] (AB75856)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PTP1B with purified ab75856 at 1/500 dilution (5.3 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Supplier Data

Western blot - Anti-PTP1B antibody [EP1841Y] (AB75856)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : PTPB1 knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab75856 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab75856 was shown to specifically react with PTP1B when PTP1B knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab75856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856)

Predicted band size: 50 kDa

false

Lab

Western blot - Anti-PTP1B antibody [EP1841Y] (AB75856)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PTP1B (phospho S378) antibody [EP1840Y] (<a href='/products/primary-antibodies/ptp1b-phospho-s378-antibody-ep1840y-ab76239'>ab76239</a>) at 1/10000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa treated with 100ng/ml Calyculin A for 10mins whole cell lysate at 15 µg

Lane 3:

HeLa treated with 100ng/ml Calyculin A for 10mins whole cell lysate, then the membrane treated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

false

Exposure time: 20s

Unknown

Western blot - Anti-PTP1B antibody [EP1841Y] (AB75856)

All lanes:

Western blot - Anti-PTP1B antibody [EP1841Y] (ab75856) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1841Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab75856 detects both phosphorylated and nonphosphorylated PTP1B.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

style

left-column

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.

Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

See full target information PTPN1

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 11:7639-7662 PubMed31527306

2019

miR-125a-5p inhibits tumorigenesis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xu,Yuquan Tao,Yongjie Niu,Zhixian Wang,Congcong Zhang,Yongchun Yu,Lifang Ma

International journal of pharmaceutics 565:391-408 PubMed31085260

2019

Optimization of miRNA delivery by using a polymeric conjugate based on deoxycholic acid-modified polyethylenimine.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Radmanesh,Hamid Sadeghi Abandansari,Sara Pahlavan,Mehdi Alikhani,Mahdi Karimi,Sarah Rajabi,Bahram Kazemi,Hossein Baharvand

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen

Scientific reports 6:36142 PubMed27824079

2016

Mena dysregulates cortactin phosphorylation to promote invadopodium maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Maxwell D Weidmann,Chinmay R Surve,Robert J Eddy,Xiaoming Chen,Frank B Gertler,Ved P Sharma,John S Condeelis

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTP1B 抗体 [EP1841Y]

Anti-PTP1B antibody [EP1841Y]

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (AB75856)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EP1841Y] (AB75856)

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EP1841Y] (AB75856)

Western blot - Anti-PTP1B antibody [EP1841Y] (AB75856)

Western blot - Anti-PTP1B antibody [EP1841Y] (AB75856)

Western blot - Anti-PTP1B antibody [EP1841Y] (AB75856)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise