JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244328

Anti-PTP1B 抗体

Anti-PTP1B antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal PTP1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PTPN1 aa 250 to C-terminus.

別名を表示する

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)

Formalin-fixed, paraffin-embedded human lung tissue stained for PTP1B with ab244328 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for PTP1B with ab244328 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)

Formalin-fixed, paraffin-embedded human placenta tissue stained for PTP1B with ab244328 at a 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody (AB244328)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody (AB244328)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling PTP1B using ab244328 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody (AB244328)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for PTP1B with ab244328 at a 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-PTP1B antibody (AB244328)
  • WB

Unknown

Western blot - Anti-PTP1B antibody (AB244328)

All lanes:

Western blot - Anti-PTP1B antibody (ab244328) at 0.4 µg/mL

Lane 1:

Control siRNA transfected U-138MG cell lysate

Lane 2:

siRNA#1 transfected U-138MG cell lysate

Lane 3:

siRNA#2 transfected U-138MG cell lysate

Predicted band size: 50 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human PTPN1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P18031

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Pharmacogenomics and personalized medicine 16:1041-1053 PubMed38058295

2023

Identification of P3H1 as a Predictive Prognostic Biomarker for Bladder Urothelial Carcinoma Based on the Cancer Genome Atlas Database.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfeng Zhang,Yang Chen,Zhiming Chen,Xinye Zhou,Shaochuan Chen,Kaijian Lan,Zhiping Wang,Yonghai Zhang
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB244207

Anti-PTP1B antibody [EPR22474]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] (AB244207)

  • 0
  • 0
Primary Antibodies

AB76239

Anti-PTP1B (phospho S378) antibody [EP1840Y]

BOND RX™ Validated|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B (phospho S378) antibody [EP1840Y] (AB76239)

  • 0
  • 0
Primary Antibodies

AB232050

Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (AB232050)

  • 0
  • 0
Primary Antibodies

AB133244

Anti-PTP1B antibody [EPR1836(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTP1B antibody [EPR1836(2)] (AB133244)

  • 5
  • 1
Primary Antibodies

AB245984

Anti-PTP1B antibody [EPR22474] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (AB245984)

  • 0
  • 0
Primary Antibodies

AB75856

Anti-PTP1B antibody [EP1841Y]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (AB75856)

  • 0
  • 0
Primary Antibodies

AB238956

Anti-PTP1B antibody [EP1841Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] - BSA and Azide free (AB238956)

  • 0
  • 0

組合せ製品

Assay Kits

AB139465

PTP1B Inhibitor Screening Assay Kit

Functional Studies - PTP1B Inhibitor Screening Assay Kit (AB139465)

  • 0
  • 0
Primary Antibodies

AB129070

Anti-PTP1B + TCPTP antibody [EPR6712]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-PTP1B + TCPTP antibody [EPR6712] (AB129070)

  • 0
  • 0
Primary Antibodies

AB32199

Anti-PTEN antibody [Y184]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-PTEN antibody [Y184] (AB32199)

  • 5
  • 15
Primary Antibodies

AB16030

Anti-SOCS3 antibody

Western blot - Anti-SOCS3 antibody (AB16030)

  • 4
  • 31
Primary Antibodies

AB154193

Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com