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リコンビナントRabMAb

Anti-PTP zeta/ Phosphacan 抗体 [EPR26271-75] (ab302790)

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Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)
  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26271-75] to PTP zeta/ Phosphacan
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PTP zeta/ Phosphacan antibody [EPR26271-75]
    PTP zeta/ Phosphacan 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26271-75] to PTP zeta/ Phosphacan

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat and mouse P14 brain tissue lysate untreated and treated with chondroitinase ABC; human, rat and mouse cerebellum, and hypothalamus tissue lysates; human and rat spinal cord tissue lysates. IHC-p: Mouse, rat, and human cerebrum FFPE tissue sections.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302790の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 500,380,300,250,180,90 kDa (predicted molecular weight: 255 kDa).
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 500,380,300,250,180,90 kDa (predicted molecular weight: 255 kDa).
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    May be involved in the regulation of specific developmental processes in the CNS.

  • 組織特異性

    Specifically expressed in the central nervous system, where it is localized in the Purkinje cell layer of the cerebellum, the dentate gyrus, and the subependymal layer of the anterior horn of the lateral ventricle. Developmentally regulated in the brain.

  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.
    Contains 1 alpha-carbonic anhydrase domain.
    Contains 1 fibronectin type-III domain.
    Contains 2 tyrosine-protein phosphatase domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 3F8 chondroitin sulfate proteoglycan antibody
    • 3H1 keratan sulfate proteoglycan antibody
    • HPTPZ antibody
    • HPTPzeta antibody
    • Phosphacan antibody
    • Protein tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • Protein tyrosine phosphatase, receptor type, Z polypeptide 1 antibody
    • Protein tyrosine phosphatase, receptor type, zeta polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • PTP-ZETA antibody
    • PTP18 antibody
    • PTPRZ antibody
    • PTPRZ_HUMAN antibody
    • Ptprz1 antibody
    • PTPZ antibody
    • R PTP zeta 2 antibody
    • R-PTP-zeta antibody
    • R-PTP-zeta-2 antibody
    • Receptor type tyrosine phosphatase beta/zeta antibody
    • Receptor-type tyrosine-protein phosphatase zeta antibody
    • RPTP-BETA antibody
    • RPTPB antibody
    • RPTPbeta antibody
    see all

画像

  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    All lanes : Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790) at 1/1000 dilution

    Lane 1 : Untreated mouse P14 brain tissue lysate
    Lane 2 : Mouse P14 brain tissue lysate treated with chondroitinase ABC
    Lane 3 : Untreated rat brain tissue lysate
    Lane 4 : Rat brain tissue lysate treated with chondroitinase ABC

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 255 kDa
    Observed band size: 180,380,500,90 kDa why is the actual band size different from the predicted?


    Exposure time: 92 seconds


    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    PTPRZ gene generates three isoforms by alternative splicing: two transmembrane isoforms PTPRZ-A and PTPRZ-B, and one secretory isoform PTPRZ-S which is also known as phosphacan. All three isoforms expressed in the brain are highly glycosylated with chondroitin sulfate. After chondroitinase ABC treatment, several bands in the range from 380 to 90 kDa were observed. The molecular weight observed is consistent with what has been described in the literature (PMID:18713734).

  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)
    Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)
    All lanes : Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790) at 1/1000 dilution

    Lane 1 : Rat spinal cord tissue lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Rat lung tissue lysate
    Lane 4 : Human cerebellum tissue lysate
    Lane 5 : Human spinal cord tissue lysate
    Lane 6 : Human liver tissue lysate
    Lane 7 : Human lung tissue lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 255 kDa
    Observed band size: 180,250,300,380,500,90 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    PTP zeta/Phosphacan are highly glycosylated with chondroitin sulfate. The molecular weight observed is consistent with what has been described in the literature (PMID:18713734).

    Negative control: liver, lung (PMID:7477903).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    All lanes : Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790) at 1/1000 dilution

    Lane 1 : Rat cerebellum tissue lysate
    Lane 2 : Rat hypothalamus tissue lysate
    Lane 3 : Mouse hippocampus tissue lysate
    Lane 4 : Mouse cerebellum tissue lysate
    Lane 5 : Mouse hypothalamus tissue lysate
    Lane 6 : Mouse liver tissue lysate
    Lane 7 : Mouse lung tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 255 kDa
    Observed band size: 500 kDa why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    PTP zeta/ Phosphacan are highly glycosylated with chondroitin sulfate. The molecular weight observed is consistent with what has been described in the literature (PMID:18713734).

    Negative controls: liver, lung (PMID:7477903)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 dilution (4.95 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID:26277687). The section was incubated with ab302790 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 dilution (4.95 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab302790 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 dilution (4.95 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab302790 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 dilution (4.95 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Negative control: No staining on mouse liver. The section was incubated with ab302790 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
    Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302790 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302790 は論文での使用が確認できていません。

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