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リコンビナントRabMAb

Anti-PTP zeta/ Phosphacan 抗体 [EPR26270-86] (ab290640)

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Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
  • Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26270-86] to PTP zeta/ Phosphacan
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PTP zeta/ Phosphacan antibody [EPR26270-86]
    PTP zeta/ Phosphacan 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26270-86] to PTP zeta/ Phosphacan

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,Flow Cyt (Intra),ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: rat cerebrum tissue, mouse cerebrum tissue, human astrocytoma tissue, human cerebrum tissue WB: rat brain tissue, rat brain tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37C, rat spinal cord tissue, mouse P14 tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37C , mouse P14 brain tissue lysate, human cerebellum tissue lysate, human spinal cord tissue lysate, NCI-H69 (human small cell lung cancer ) whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290640の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/2000.
WB
1/1000. Predicted molecular weight: 255 kDa.
特記事項
IHC-P
1/2000.
WB
1/1000. Predicted molecular weight: 255 kDa.
追加情報
Is unsuitable for Flow Cyt,Flow Cyt (Intra),ICC/IF,IHC-Fr or IP.

ターゲット情報

  • 機能

    May be involved in the regulation of specific developmental processes in the CNS.

  • 組織特異性

    Specifically expressed in the central nervous system, where it is localized in the Purkinje cell layer of the cerebellum, the dentate gyrus, and the subependymal layer of the anterior horn of the lateral ventricle. Developmentally regulated in the brain.

  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.
    Contains 1 alpha-carbonic anhydrase domain.
    Contains 1 fibronectin type-III domain.
    Contains 2 tyrosine-protein phosphatase domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 3F8 chondroitin sulfate proteoglycan antibody
    • 3H1 keratan sulfate proteoglycan antibody
    • HPTPZ antibody
    • HPTPzeta antibody
    • Phosphacan antibody
    • Protein tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • Protein tyrosine phosphatase, receptor type, Z polypeptide 1 antibody
    • Protein tyrosine phosphatase, receptor type, zeta polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • PTP-ZETA antibody
    • PTP18 antibody
    • PTPRZ antibody
    • PTPRZ_HUMAN antibody
    • Ptprz1 antibody
    • PTPZ antibody
    • R PTP zeta 2 antibody
    • R-PTP-zeta antibody
    • R-PTP-zeta-2 antibody
    • Receptor type tyrosine phosphatase beta/zeta antibody
    • Receptor-type tyrosine-protein phosphatase zeta antibody
    • RPTP-BETA antibody
    • RPTPB antibody
    • RPTPbeta antibody
    see all

画像

  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    All lanes : Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37?
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Rat spinal cord tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 255 kDa



    Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

    PTPRZ gene generates three isoforms by alternative splicing: two transmembrane isoforms PTPRZ-A and PTPRZ-B, and one secretory isoform PTPRZ-S which is also known as phosphacan. All three isoforms expressed in the brain are highly glycosylated with chondroitin sulfate. After chondroitinase ABC treatment, several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID:18713734).

    Negative control: liver(PMID:7477903).

  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    All lanes : Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640) at 1/1000 dilution

    Lane 1 : Human cerebellum tissue lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Human spinal cord tissue lysate
    Lane 4 : NCI-H69 (human small cell lung cancer ) whole cell lysate
    Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 255 kDa
    Observed band size: 380-70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

    Several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID:18713734).

    Negative control: liver, U-87 MG(PMID:7477903, 23170925).

  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    All lanes : Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640) at 1/1000 dilution

    Lane 1 : Mouse P14 brain tissue lysate
    Lane 2 : Mouse P14 brain tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37?
    Lane 3 : Mouse liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 255 kDa
    Observed band size: 380-70 kDa why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

    After chondroitinase ABC treatment, several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID:18713734).

    Negative control: liver(PMID:7477903).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)

    Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human astrocytoma. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Positive staining on mouse cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Positive staining on rat cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Negative control: no staining on human liver. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)
    Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab290640 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab290640 は論文での使用が確認できていません。

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