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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PTN 抗体 [EPR3041] (ab79411)

Submit a review Submit a question References (8)
Western blot - Anti-PTN antibody [EPR3041] (ab79411)
  • Immunoprecipitation - Anti-PTN antibody [EPR3041] (ab79411)
  • Western blot - Anti-PTN antibody [EPR3041] (ab79411)
  • Western blot - Anti-PTN antibody [EPR3041] (ab79411)
  • Anti-PTN antibody [EPR3041] (ab79411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3041] to PTN
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PTN antibody [EPR3041]
    PTN 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3041] to PTN

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human brain, Mouse brain, Mouse cerebellum, and Rat brain tissue lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab79411の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

For unpurified use at 1/10000 - 1/20000.
特記事項
WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

For unpurified use at 1/10000 - 1/20000.

ターゲット情報

  • 機能

    Heparin binding mitogenic protein. Has neurite extension activity.

  • 組織特異性

    Osteoblast and brain.

  • 配列類似性

    Belongs to the pleiotrophin family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • HARP antibody
    • HB-GAM antibody
    • HBBM antibody
    • HBGAM antibody
    • HBGF-8 antibody
    • HBGF8 antibody
    • HBNF antibody
    • HBNF-1 antibody
    • HBNF1 antibody
    • heparin affin regulatory protein antibody
    • Heparin binding growth associated molecule antibody
    • Heparin binding growth factor 8 antibody
    • Heparin binding neurite outgrowth promoting factor 1 antibody
    • Heparin-binding brain mitogen antibody
    • Heparin-binding growth factor 8 antibody
    • Heparin-binding growth-associated molecule antibody
    • heparin-binding neurite outgrowth promoting factor antibody
    • Heparin-binding neurite outgrowth-promoting factor 1 antibody
    • NEGF1 antibody
    • Neurite growth promoting factor 1 antibody
    • Neurite outgrowth-promoting factor, heparin-binding antibody
    • OSF-1 antibody
    • OSF1 antibody
    • Osteoblast-specific factor 1 antibody
    • pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) antibody
    • Pleiotrophin antibody
    • PTN antibody
    • PTN_HUMAN antibody
    see all

画像

  • Western blot - Anti-PTN antibody [EPR3041] (ab79411)
    Western blot - Anti-PTN antibody [EPR3041] (ab79411)
    All lanes : Anti-PTN antibody [EPR3041] (ab79411) at 1/500 dilution

    Lane 1 : Wild-type U87mg lysate
    Lane 2 : PTN Knockout U87mg lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    ab79411 was shown to react with PTN in wild-type U87mg cells in Western blot with loss of signal observed in PTN knockout cell line ab288713. Wild-type U87mg and PTN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab79411 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
  • Immunoprecipitation - Anti-PTN antibody [EPR3041] (ab79411)
    Immunoprecipitation - Anti-PTN antibody [EPR3041] (ab79411)
    Immunoprecipitation of PTN in U87mg cells. Lysates were prepared and immunoprecipitation was performed using 2.0 µg of ab79411 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
  • Western blot - Anti-PTN antibody [EPR3041] (ab79411)
    Western blot - Anti-PTN antibody [EPR3041] (ab79411)
    All lanes : Anti-PTN antibody [EPR3041] (ab79411) at 1/2000 dilution (Purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse cerebellum lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Western blot - Anti-PTN antibody [EPR3041] (ab79411)
    Western blot - Anti-PTN antibody [EPR3041] (ab79411)
    Anti-PTN antibody [EPR3041] (ab79411) at 1/1000 dilution (Purified) + Human brain lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Anti-PTN antibody [EPR3041] (ab79411)
    Anti-PTN antibody [EPR3041] (ab79411)

参考文献 (8)

ab79411 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab79411 は 8 報の論文で使用されています。

  • Qiu X  et al. Pleiotrophin ameliorates white matter injury of neonatal rats by activating the mTOR/YY1/Id4 signaling pathway. FASEB J 37:e23082 (2023). PubMed: 37462506
  • Chen P  et al. NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage. J Neuroinflammation 20:261 (2023). PubMed: 37953259
  • Jia B  et al. CircRNA-PTN Sponges miR-326 to Promote Proliferation in Hepatocellular Carcinoma. Onco Targets Ther 13:4893-4903 (2020). PubMed: 32581550
  • Huang P  et al. Chemotherapy-driven increases in the CDKN1A/PTN/PTPRZ1 axis promote chemoresistance by activating the NF-?B pathway in breast cancer cells. Cell Commun Signal 16:92 (2018). PubMed: 30497491
  • Liu S  et al. Knockdown of pleiotrophin increases the risk of preeclampsia following vitrified-thawed embryo transfer. Int J Oncol 53:1847-1856 (2018). PubMed: 30226583
  • Zhu B  et al. Array profiling reveals contribution of Cthrc1 to growth of the denervated rat urinary bladder. Am J Physiol Renal Physiol 314:F893-F905 (2018). PubMed: 29357417
  • Kuboyama K  et al. Role of Chondroitin Sulfate (CS) Modification in the Regulation of Protein-tyrosine Phosphatase Receptor Type Z (PTPRZ) Activity: PLEIOTROPHIN-PTPRZ-A SIGNALING IS INVOLVED IN OLIGODENDROCYTE DIFFERENTIATION. J Biol Chem 291:18117-28 (2016). PubMed: 27445335
  • Kuboyama K  et al. Inactivation of Protein Tyrosine Phosphatase Receptor Type Z by Pleiotrophin Promotes Remyelination through Activation of Differentiation of Oligodendrocyte Precursor Cells. J Neurosci 35:12162-71 (2015). PubMed: 26338327

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