AB23668

Anti-PTGIS/PGIS 抗体

Name: Anti-PTGIS/PGIS 抗体 (ab23668) | Abcam
Brand: Abcam Limited
SKU: AB23668
Price: 75000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-PTGIS/PGIS antibody

(2 Reviews)

(19 Publications)

Rabbit Polyclonal PTGIS/PGIS antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human, Cow, Sheep samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ptgis aa 450-500.

別名を表示する

Cyp8, Prostacyclin synthase, Hydroperoxy icosatetraenoate dehydratase, Prostaglandin I2 synthase

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (AB23668)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (AB23668)

ab23668 (4μg/ml) staining PTGIS/PGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Sheep, Cow, Rat, Mouse

アプリケーション

IHC-Fr, WB, ICC/IF, IHC-P

applications

免疫原

Synthetic Peptide within Mouse Ptgis aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

O35074

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Reactivity data

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出荷温度及び保存条件

製品の状態

Liquid

精製に関する特記事項

Affinity purified

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein PTGIS also known as prostacyclin synthase (PGIS) is an enzyme involved in the conversion of prostaglandin H2 (PGH2) to prostacyclin (PGI2) a member of the prostaglandin family. It has a molecular mass of approximately 56 kDa. PTGIS is mainly expressed in vascular endothelial cells as well as in other tissues including heart kidney and lung. The protein is important for various physiological functions due to its enzymatic role in prostacyclin synthesis.

Biological function summary

PTGIS plays a role in vasodilation and inhibiting platelet aggregation. Prostacyclin the product of PTGIS activity functions as a potent vasodilator and inhibitor of platelet activation which are key processes for maintaining vascular health. PTGIS does not form part of a larger protein complex but works independently to generate its product PGI2. By performing these roles PTGIS contributes significantly to cardiovascular homeostasis.

Pathways

PTGIS is an important component of the prostaglandin biosynthesis pathway. This pathway is important for the formation of several types of prostaglandins which are involved in various aspects of inflammation and vascular function. PTGIS connects closely with cyclooxygenase (COX) enzymes specifically COX-1 and COX-2 which generate PGH2 from arachidonic acid. The interaction with COX enzymes is necessary for the effective production of prostacyclin.

PTGIS has implications in thrombotic diseases and pulmonary hypertension. The enzyme's role in inhibiting platelet aggregation makes it relevant in conditions where clot formation is a risk such as cardiovascular disorders. Its relation to pulmonary hypertension involves dysregulation of prostacyclin production which affects blood pressure in lung arteries. Additionally interactions with proteins like thromboxane synthase which counteracts the effects of prostacyclin are important for understanding the balance between factors promoting and inhibiting thrombosis in these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).

See full target information Ptgis

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文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 13:e032872 PubMed38639351

2024

Identification of Rare Variants in Patients With Williams Syndrome and Severe Peripheral Pulmonary Stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ayako Chida-Nagai,Hiroyuki Akagawa,Saori Sawai,Yue-Jiao Ma,Satoshi Yakuwa,Jun Muneuchi,Kazushi Yasuda,Hirokuni Yamazawa,Toshiyuki Yamamoto,Emi Takakuwa,Utano Tomaru,Yoshiyuki Furutani,Tatsuya Kato,Gen Harada,Kei Inai,Toshio Nakanishi,Atsushi Manabe,Atsuhito Takeda,Zhi-Cheng Jing

Cells 13: PubMed38334598

2024

Blastocyst-Derived Lactic Acid May Regulate S100A6 Expression and Function in Mouse Decidualization via Stimulation of Uterine Epithelial Arachidonic Acid Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yuan Li,Ying Wang,Ying Wu,Xu-Yu Zhao,Zhen-Shan Yang,Bo Li,Si-Ting Chen,Yu-Ying He,Zeng-Ming Yang

Journal of the American Society of Nephrology : JASN 35:149-165 PubMed38062563

2023

Prostacyclin Mitigates Renal Fibrosis by Activating Fibroblast Prostaglandin I 2 Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Yi Guan,Yunyu Xu,Yingxue Cao,Qionghong Xie,Raymond C Harris,Matthew D Breyer,Limin Lu,Chuan-Ming Hao

JCO precision oncology 7:e2200630 PubMed37437228

2023

Key Molecules in Bladder Cancer Affect Patient Prognosis and Immunotherapy Efficacy: Further Exploration for and .

Applications

Unspecified application

Species

Unspecified reactive species

Tailai Zhou,Hengxin Chen,Yinzhao Wang,Sijie Wen,Pinghong Dao,Minfeng Chen

Physiological reports 10:e15505 PubMed36324300

2022

Identification of exercise-regulated genes in mice exposed to cigarette smoke.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Aakerøy,Chew W Cheng,Pavla Sustova,Nathan R Scrimgeour,Sissel Gyrid Freim Wahl,Sigurd Steinshamn,T Scott Bowen,Eivind Brønstad

Frontiers in endocrinology 13:942368 PubMed36339397

2022

Identification and analysis of novel endometriosis biomarkers integrative bioinformatics.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Jin Bae,Yunju Jo,Min Kyoung Cho,Jung-Sook Jin,Jin-Young Kim,Jaewon Shim,Yun Hak Kim,Jang-Kyung Park,Dongryeol Ryu,Hyun Joo Lee,Jongkil Joo,Ki-Tae Ha

Frontiers in oncology 11:719564 PubMed34722263

2021

Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Su,Yufang Lv,Wenhao Lu,Zhenyuan Yu,Yu Ye,Bingqian Guo,Deyun Liu,Haibiao Yan,Tianyu Li,Qingyun Zhang,Jiwen Cheng,Zengnan Mo

Animals : an open access journal from MDPI 11: PubMed34573602

2021

Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona Kowalczyk-Zieba,Joanna Staszkiewicz-Chodor,Dorota Boruszewska,Krzysztof Lukaszuk,Joanna Jaworska,Izabela Woclawek-Potocka

Laboratory investigation; a journal of technical methods and pathology 101:1210-1224 PubMed34112940

2021

Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Yin Pan,Ling Wang,Hong-Mei You,Miao Cheng,Yang Yang,Cheng Huang,Jun Li

The EMBO journal 39:e103454 PubMed32484988

2020

Tissue-resident macrophages actively suppress IL-1beta release via a reactive prostanoid/IL-10 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Natacha Ipseiz,Robert J Pickering,Marcela Rosas,Victoria J Tyrrell,Luke C Davies,Selinda J Orr,Magdalena A Czubala,Dina Fathalla,Avril Ab Robertson,Clare E Bryant,Valerie O'Donnell,Philip R Taylor

View all publications

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Abcam product promise

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Anti-PTGIS/PGIS 抗体

Anti-PTGIS/PGIS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (AB23668)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製に関する特記事項

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (19)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise