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リコンビナントRabMAb

Anti-PTGES2/Gbf1 抗体 [EPR25169-3] (ab300052)

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Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
  • Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
  • Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25169-3] to PTGES2/Gbf1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PTGES2/Gbf1 antibody [EPR25169-3]
    PTGES2/Gbf1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25169-3] to PTGES2/Gbf1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human heart, Human kidney, Mouse heart, Mouse kidney, Mouse thymus, Rat heart and Rat lung lysates. IHC-P: Human kidney, Human colon carcinoma, Mouse cardiac muscle and Rat cardiac muscle tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25169-3

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300052の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 30, 30, 45 kDa (predicted molecular weight: 42 kDa).
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 30, 30, 45 kDa (predicted molecular weight: 42 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

ターゲット情報

  • 機能

    Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear.

  • 組織特異性

    Widely expressed. Expressed in the heart, including apex, inter-ventricular septum, both atria and ventricles, but not in the aorta. Also expressed in fetal heart. Detected in various regions of the brain: cerebellum; occipital, frontal and parietal lobes. Also expressed in the lymph nodes, skeletal muscle, kidney and trachea, but not in the thymus or lung. Overexpressed in colorectal cancer.

  • パスウェイ

    Lipid metabolism; prostaglandin biosynthesis.

  • 配列類似性

    Belongs to the GST superfamily.
    Contains 1 glutaredoxin domain.
    Contains 1 GST C-terminal domain.

  • 細胞内局在

    Golgi apparatus membrane and Cytoplasm > perinuclear region. Synthetized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. The truncated form is cytoplasmic and is enriched in the perinuclear region.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C9orf15 antibody
      • FLJ14038 antibody
      • Gamma interferon activated transcriptional element binding factor 1 antibody
      • GATE binding factor 1 antibody
      • GBF 1 antibody
      • GBF1 antibody
      • Membrane associated prostaglandin E synthase 2 antibody
      • MGC11289 antibody
      • Microsomal prostaglandin E synthase 2 antibody
      • mPGES 2 antibody
      • mPGES-2 antibody
      • PGES2 antibody
      • PGES2_HUMAN antibody
      • Prostaglandin E synthase 2 antibody
      • Prostaglandin E synthase 2 truncated form antibody
      • PTGES 2 antibody
      • PTGES2 antibody
      see all

    画像

    • Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      All lanes :

      Lane 1 : Human heart tissue lysate
      Lane 2 : Human kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 42 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      Blocking, diluting buffer and concentration was 5% NFDM/TBST. 

    • Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      All lanes : Anti-PTGES2/Gbf1 antibody [EPR25169-3] (ab300052) at 1/1000 dilution

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Mouse thymus tissue lysate
      Lane 4 : Rat heart tissue lysate
      Lane 5 : Rat lung tissue lysate

      Lysates/proteins at 1/20 dilution per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 30, 45 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      Blocking, diluting buffer and concentration was 5% NFDM/TBST. 

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 12835322).
      Low expression: mouse thymus, rat lung (PMID: 15744024, 11866447). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling PTGES2/Gbf1 with ab300052 at 1/500 (0.944 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human renal tubules. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)

      Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling PTGES2/Gbf1 with ab300052 at 1/500 (0.944 µ/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human colon carcinoma. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)

      Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling PTGES2/Gbf1 with ab300052 at 1/100 (4.72 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cardiac muscle. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)

      Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling PTGES2/Gbf1 with ab300052 at 1/100 (4.72 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cardiac muscle. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
      Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300052 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300052 は論文での使用が確認できていません。

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