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リコンビナントRabMAb

Anti-PTCH2 抗体 [EPR26073-39] - BSA and Azide free (ab300037)

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Western blot - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
  • Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
  • Flow Cytometry (Intracellular) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
  • Immunoprecipitation - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
  • Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26073-39] to PTCH2 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt (Intra), IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free
    PTCH2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26073-39] to PTCH2 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, Flow Cyt (Intra), IHC-P, ICC/IFmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: SH-SY5Y and MDA-MB-231 whole cell lysates. IHC-P: Human: testis and ovarian carcinoma tissues. ICC: SH-SY5Y cells. Flow Cyt (intra): SH-SY5Y cell. IP: SH-SY5Y cell.

  • 特記事項

    Ab300037 is a carrier free version of ab300036. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300037の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH).

  • 関連疾患

    Defects in PTCH2 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome).
    Defects in PTCH2 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].

  • 配列類似性

    Belongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • patched (Drosophila) homolog 2 antibody
      • Patched 2 antibody
      • patched antibody
      • patched homolog 2 (Drosophila) antibody
      • patched-2 antibody
      • Protein patched homolog 2 antibody
      • PTC2 antibody
      • PTC2_HUMAN antibody
      • Ptch2 antibody
      see all

    画像

    • Western blot - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Western blot - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      All lanes : Anti-PTCH2 antibody [EPR26073-39] (ab300036) at 1/1000 dilution

      Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 131 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?


      Exposure time: 158 seconds


      This data was developed using ab300036, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

      This data was developed using ab300036, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PTCH2 with ab300036 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human testis. The section was incubated with ab300036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody (ab300036) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

      This data was developed using ab300036, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling PTCH2 with ab300036 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human ovarian carcinoma. The section was incubated with ab300036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody (ab300036) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

      This data was developed using ab300036, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling PTCH2 with ab300036 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on human cardiac muscle. The section was incubated with ab300036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody (ab300036) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

      This data was developed using ab300036, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized SH-SY5Y (human neuroblastoma epithelial cell) cells lebelling PTCH2 with ab300036 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membrane and weak cytoplasm staining in SH-SY5Y cell line.

      Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594, ab195889) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Flow Cytometry (Intracellular) - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

      This data was developed using ab300036, the same antibody clone in a different buffer formulation.

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling PTCH2 with ab300036 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Immunoprecipitation - Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

      This data was developed using ab300036, the same antibody clone in a different buffer formulation.

      PTCH2 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 µg with ab300036 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab300036 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 µg (Input)

      Lane 2: ab300036 IP in SH-SY5Y whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300036 in SH-SY5Y whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 32 seconds

      Lysates were made freshly and used in IP test immediately to minimize protein degradation.

    • Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)
      Anti-PTCH2 antibody [EPR26073-39] - BSA and Azide free (ab300037)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300037 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300037 は論文での使用が確認できていません。

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