Anti-PTBP2 抗体 [EPR9891] (ab154853)
![Western blot - Anti-PTBP2 antibody [EPR9891] (ab154853)](https://www.abcam.co.jp/ps/products/154/ab154853/Images/ab154853-161470-anti-ptbp2-antibody-epr9891-western-blot.jpg "Western blot - Anti-PTBP2 antibody [EPR9891] (ab154853)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9891] to PTBP2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PTBP2 antibody [EPR9891]
PTBP2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9891] to PTBP2 -
由来種
Rabbit -
特異性
The immunogen used for this product shares 93% homology with PTBP1 and PTBP3. Cross-reactivity with this protein has not been confirmed experimentally.
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アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal brain, Y79 and Neuro 2a lysates; Y79 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9891 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. -
組織特異性
Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues. -
配列類似性
Contains 4 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 58155 Human
- Entrez Gene: 56195 Mouse
- Entrez Gene: 310820 Rat
- Omim: 608449 Human
- SwissProt: Q9UKA9 Human
- SwissProt: Q91Z31 Mouse
- SwissProt: Q66H20 Rat
- Unigene: 269895 Human
see all -
別名
- brPTB antibody
- FLJ34897 antibody
- MIBP antibody
see all
画像
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Western blot - Anti-PTBP2 antibody [EPR9891] (ab154853)All lanes : Anti-PTBP2 antibody [EPR9891] (ab154853) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 cell lysate
Lane 3 : Neuro 2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 57 kDa -
![Flow Cytometry (Intracellular) - Anti-PTBP2 antibody [EPR9891] (ab154853)](https://www.abcam.co.jp/ps/products/154/ab154853/Images/ab154853-161469-anti-ptbp2-antibody-epr9891-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PTBP2 antibody [EPR9891] (ab154853)Intracellular flow cytometric analysis of permeabilized Y79 cells labeling PTBP2 with ab154853 at 1/100 dilution (red). Rabbit IgG negative control (green).
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![Anti-PTBP2 antibody [EPR9891] (ab154853)](https://www.abcam.co.jp/ps/products/154/ab154853/Images/ab154853-3-benefits-of-recombinant-antibodies.png)
Anti-PTBP2 antibody [EPR9891] (ab154853)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154853 は 3 報の論文で使用されています。
- Kim JJ et al. Dynamics of TERT regulation via alternative splicing in stem cells and cancer cells. PLoS One 18:e0289327 (2023). PubMed: 37531400
- Sayed ME et al. NOVA1 directs PTBP1 to hTERT pre-mRNA and promotes telomerase activity in cancer cells. Oncogene 38:2937-2952 (2019). PubMed: 30568224
- Tahmasebi S et al. Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2. Proc Natl Acad Sci U S A 113:12360-12367 (2016). PubMed: 27791185