Anti-PTBP1 抗体 (ab154887)
Key features and details
- Rabbit polyclonal to PTBP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PTBP1 antibody
PTBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PTBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Pig, Xenopus tropicalis
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免疫原
Recombinant fragment corresponding to a region within amino acids 293-528 of Human PTBP1 (UniProt ID: P26599).
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ポジティブ･コントロール
- 293T, A431, HeLa and HepG2 whole cell lysate. Human AGS xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154887の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. -
配列類似性
Contains 4 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 420098 Chicken
- Entrez Gene: 282018 Cow
- Entrez Gene: 5725 Human
- Entrez Gene: 19205 Mouse
- Entrez Gene: 397461 Pig
- Entrez Gene: 29497 Rat
- Entrez Gene: 496557 Xenopus tropicalis
- Omim: 600693 Human
see all -
別名
- 57 kDa RNA binding protein PPTB 1 antibody
- 57 kDa RNA-binding protein PPTB-1 antibody
- Heterogeneous nuclear ribonucleoprotein I antibody
see all
画像
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Western blot - Anti-PTBP1 antibody (ab154887)All lanes : Anti-PTBP1 antibody (ab154887) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab154887)Immunohistochemical analysis of paraffin embedded Human AGS xenograft tissue, labeling PTBP1 using ab154887 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154887 は論文での使用が確認できていません。