AB214012

Anti-PSPC1 抗体 [1L4] - C-terminal

Name: Anti-PSPC1 抗体 [1L4] - C-terminal (ab214012) | Abcam
Brand: Abcam Limited
SKU: AB214012
Price: 71000 JPY
Availability: InStock

Anti-PSPC1 antibody [1L4] - C-terminal

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(1 Publication)

Mouse Monoclonal PSPC1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Pspc1 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

PSP1, PSPC1, Paraspeckle component 1, Paraspeckle protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

Immunofluorescent analysis of HeLa cells,4% paraformaldehyde fixed and 0.5% Triton X-100 permeabilized, labeling PSPC1 with ab214012 at 5 μg/mL dilution. Secondary antibody : Goat Anti-Mouse IgG, Cy3 conjugate.

Supplier Data

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

Lane 1:

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 0.5 µg/mL

Lane 2:

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 1 µg/mL

Lane 3:

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 2 µg/mL

All lanes:

Hepa1-6 whole cell lysate

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase

Predicted band size: 59 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

1L4

アイソタイプ

IgG1

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, WB

applications

免疫原

Synthetic Peptide within Mouse Pspc1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8R326

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

精製に関する特記事項

Purified Immunoglobulin

バッファー組成

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PSPC1 also known as Paraspeckle Component 1 is a nuclear RNA-binding protein with a mass of approximately 53 kDa. This protein plays an important mechanical role in the formation of paraspeckles subnuclear structures involved in RNA processing and regulation. PSPC1 predominantly localizes in the nucleus and associates with non-coding RNAs such as NEAT1 contributing to nuclear body dynamics. Though present in several tissues its expression is notably high in testis and embryonic stem cells reflecting its roles in cellular processes.

Biological function summary

PSPC1 participates in the regulation of gene expression by modulating the transcriptional activity and stability of specific RNAs. It functions as part of a larger RNA-protein complex that involves core paraspeckle proteins such as NONO and SFPQ. This complex helps in retaining hyper-edited RNAs within the nucleus preventing them from undergoing translation or degradation. By doing so PSPC1 influences cellular stress responses and development among other biological processes.

Pathways

PSPC1 integrates into the cellular landscape by impacting the RNA processing and nuclear organization pathways. It maintains RNA homeostasis and transcript diversity acting synergistically with related proteins like NONO and SFPQ. Within the context of these pathways paraspeckle-associated activities involve RNA editing and sequestration linking PSPC1 to critical cellular functions such as transcriptional regulation and response to environmental changes.

PSPC1's association with various conditions occurs through its regulatory mechanisms in gene expression. Aberrations in PSPC1 expression or function may contribute to cancer progression particularly in prostate cancer by influencing oncogenic signaling pathways. Moreover its interactions with other proteins such as NONO can impact neurodegenerative disorders through altered RNA metabolism and neuronal gene expression. Understanding PSPC1's roles aids in developing targeted therapies for these conditions by modulating its function or associated pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

RNA-binding protein required for the formation of nuclear paraspeckles (PubMed : 22416126). Binds to poly(A), poly(G) and poly(U) RNA homopolymers (PubMed : 22416126). Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728).

See full target information PSPC1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine : PubMed33169942

2020

Metformin attenuates trauma-induced heterotopic ossification via inhibition of Bone Morphogenetic Protein signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Lin,Fuli Shi,Shanshan Jiang,Yuanyuan Wang,Junrong Zou,Ying Ying,Deqiang Huang,Lingyu Luo,Xiaohua Yan,Zhijun Luo

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSPC1 抗体 [1L4] - C-terminal

Anti-PSPC1 antibody [1L4] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製度

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise