AB140497

Anti-PSMF1 抗体

Name: Anti-PSMF1 抗体 (ab140497) | Abcam
Brand: Abcam Limited
SKU: AB140497
Price: 76000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-PSMF1 antibody

(1 Review)

(2 Publications)

Rabbit Polyclonal PSMF1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Proteasome inhibitor PI31 subunit aa 200 to C-terminus.

別名を表示する

Proteasome inhibitor PI31 subunit, hPI31, PSMF1

2 Images

Unknown

Immunoprecipitation - Anti-PSMF1 antibody (AB140497)

ab140497 at 1 μg/ml detecting PSMF1 in 293T whole cell lysate by WB following IP.

Lane 1 : ab140497 at 6μg/mg of lysate
Lane 2 : control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PSMF1 antibody (ab140497)

Predicted band size: 30 kDa

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Unknown

Western blot - Anti-PSMF1 antibody (AB140497)

All lanes:

Western blot - Anti-PSMF1 antibody (ab140497) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 30 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human Proteasome inhibitor PI31 subunit aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92530

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab140497 was affinity purified using an epitope specific to PSMF1 immobilized on a solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PSMF1 also known as proteasome inhibitor subunit 1 or PI31 inhibits the proteasome a protein complex responsible for degrading unwanted or damaged proteins through ubiquitin-proteasome pathway. PSMF1 has a mass of approximately 29 kDa and shows expression in a variety of human tissues with notable presence in the liver brain and testis. Its role as an inhibitor allows regulation of proteasomal activity playing important part in maintaining protein homeostasis.

Biological function summary

PSMF1 interacts with proteasomal subunits functioning within the core particle of the 20S proteasome complex. Its inhibitory action helps balance protein degradation in cells impacting processes such as cell cycle apoptosis and stress responses. The modulating effect of PSMF1 on the proteasome distinguishes its role in cellular regulatory mechanisms.

Pathways

PSMF1 contributes to the ubiquitin-proteasome pathway and influences the NF-kB signaling pathway. PSMF1's interactions within this system connect it with other essential proteins including ubiquitin which mark proteins for degradation and the proteasome the complex where degradation occurs. This process affects the transcription factor NF-kB which regulates genes involved in immune response and inflammation.

PSMF1 has been associated with cancer and neurodegenerative diseases like Parkinson’s disease. In cancer its role in regulating proteasome activity impacts cellular proliferation and survival connecting it to other proteins such as p53 involved in tumor suppression. In neurodegenerative disorders impaired proteasome function links PSMF1 to the accumulation of misfolded proteins with proteins like alpha-synuclein involved in the pathology of conditions such as Parkinson’s disease.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.

See full target information Proteasome inhibitor PI31 subunit

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:3928-3945 PubMed35664068

2022

5-HT2B-mediated serotonin activation in enterocytes suppresses colitis-associated cancer initiation and promotes cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Mao,Fang Xin,Jie Ren,Shuai Xu,Haixia Huang,Xu Zha,Xinxin Wen,Guoqing Gu,Guang Yang,Yuan Cheng,Chen Zhang,Wei Wang,Xicheng Liu

Developmental cell 50:509-524.e10 PubMed31327739

2019

PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Sandra Jones,Adi Minis,Jose Rodriguez,Henrik Molina,Hermann Steller

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMF1 抗体

Anti-PSMF1 antibody

Immunoprecipitation - Anti-PSMF1 antibody (AB140497)

Western blot - Anti-PSMF1 antibody (AB140497)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise