AB109130

Anti-PSMD14 抗体 [EPR4258]

Anti-PSMD14 antibody [EPR4258]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal PSMD14 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

別名を表示する

POH1, PSMD14, Ubiquitin C-terminal hydrolase PSMD14, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1

2 Images

Lab

Immunoprecipitation - Anti-PSMD14 antibody [EPR4258] (AB109130)

PSMD14 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg with 109130 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg

Lane 2 : ab109130 IP in K-562 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab109130 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PSMD14 antibody [EPR4258] (ab109130)

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Unknown

Western blot - Anti-PSMD14 antibody [EPR4258] (AB109130)

All lanes:

Western blot - Anti-PSMD14 antibody [EPR4258] (ab109130) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

Namalwa cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Lane 6:

MCF7 cell lysate at 10 µg

Predicted band size: 35 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4258

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PSMD14 also known as POH1 or RPN11 is a proteasome subunit involved in the regulation of protein degradation. This protein has a molecular mass of approximately 34 kDa and forms an essential part of the 19S regulatory particle of the proteasome complex. PSMD14 is expressed in various tissues but shows significant expression levels in the brain heart and skeletal muscles. By deubiquitinating substrates PSMD14 contributes to the recycling of ubiquitin for further use in the cell’s ubiquitin-proteasome pathway.

Biological function summary

PSMD14 functions as a vital component of the 19S proteasome which is integrated into the proteolytic 26S proteasome complex. The protein's deubiquitinating activity plays a role in maintaining proteostasis by removing ubiquitin chains from substrates destined for degradation. This process is important for regulating protein quality control cell cycle progression and response to oxidative stress among other cellular processes. As part of the proteasome complex PSMD14’s activity impacts cellular homeostasis and contributes to cellular responses to various stimuli.

Pathways

PSMD14 is instrumental in the ubiquitin-proteasome system which is essential for targeted protein degradation modulation of the cell cycle and regulation of transcription factors. It interacts with other proteasome components like PSMD12 and PSMD3 to form a functional proteasome unit. This protein is significant in the NF-kB signaling pathway where it influences the degradation of inhibitor proteins allowing for the activation of NF-kB a critical transcription factor in immune response and cell survival.

PSMD14’s function connects it to cancer and neurodegenerative conditions. Deregulation or mutations in PSMD14 have been implicated in various cancers possibly through disturbed protein degradation which leads to aberrant cell cycle progression and survival. It also relates to neurodegenerative diseases like Alzheimer's as impaired proteasome function can lead to the accumulation of misfolded proteins. PSMD14 interacts with proteins like UCH37 which is also linked to the pathogenesis of these diseases highlighting the significance of controlled proteolysis in maintaining cellular health.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed : 9374539, PubMed : 1317798). The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex (PubMed : 22909820). Plays a role in response to double-strand breaks (DSBs) : acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation (PubMed : 22909820). Also involved in homologous recombination repair by promoting RAD51 loading (PubMed : 22909820). Regulates macroautophagy by ensuring Golgi-to-ER retrograde transport through its deubiquitinating activity on K63-linked ubiquitin chains. This activity prevents the retention of essential autophagy proteins at the Golgi, enabling their trafficking to autophagosome formation sites and supporting Golgi-ER membrane recycling critical for effective autophagy (PubMed : 32210007).

See full target information PSMD14

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

The Kaohsiung journal of medical sciences 41:e70007 PubMed40066751

2025

PSMD14 Transcriptionally Activated by MEF2A Promotes Pancreatic Cancer Development by Upregulating SPON2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-He Hao,Cheng-Ru Yang,Wu-Jiang Shi,Xiang-Yu Zhong

Journal of thoracic disease 15:3885-3907 PubMed37559628

2023

Bioinformatics analysis and single-cell RNA sequencing: elucidating the ubiquitination pathways and key enzymes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Lu,Ran Xu,Chenghao Wang,Xiang Zhou,Rafael Parra-Medina,Roberto Díaz-Peña,Bo Peng,Linyou Zhang

Brain sciences 12: PubMed35884735

2022

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Zhang,Chenyu Zhang,Cong Liu,Haohong Zhan,Bo Li,Yuanzhen Lu,Hongyan Wei,Jingge Cheng,Shuhao Li,Chuyue Wang,Chunlin Hu,Xiaoxing Liao

eLife 11: PubMed35451368

2022

A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiale Du,Sandor Babik,Yanfeng Li,Kirandeep K Deol,Stephen J Eyles,Jasna Fejzo,Marco Tonelli,Eric Strieter

Molecular cell 80:796-809.e9 PubMed33156996

2020

Proteasome-Bound UCH37/UCHL5 Debranches Ubiquitin Chains to Promote Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Kirandeep K Deol,Sean O Crowe,Jiale Du,Heather A Bisbee,Robert G Guenette,Eric R Strieter

Cell host & microbe 24:761-775.e6 PubMed30503508

2018

Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Fletcher,Marina Vaysburd,Sarah Maslen,Jingwei Zeng,J Mark Skehel,Greg J Towers,Leo C James

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMD14 抗体 [EPR4258]

Anti-PSMD14 antibody [EPR4258]

Immunoprecipitation - Anti-PSMD14 antibody [EPR4258] (AB109130)

Western blot - Anti-PSMD14 antibody [EPR4258] (AB109130)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

Abcam product promise