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AB109123

Anti-PSMD14 抗体 [EPR4257]

Anti-PSMD14 antibody [EPR4257]

5

(2 Reviews)

|

(5 Publications)

Rabbit Recombinant Monoclonal PSMD14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

別名を表示する

POH1, PSMD14, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1

3 Images
Western blot - Anti-PSMD14 antibody [EPR4257] (AB109123)
  • WB

Unknown

Western blot - Anti-PSMD14 antibody [EPR4257] (AB109123)

All lanes:

Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution

Lane 1:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 4:

Namalwa (Burkitt's lymphoma cell line) whole cell lysates at 10 µg

Lane 5:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 35 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (AB109123)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (AB109123)

ab109123 at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (AB109123)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (AB109123)

ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-PSMD14 antibody [EPR4257] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4257

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p>(Heat to 98°C allow to cool for 10-20 min).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PSMD14 also known as POH1 or RPN11 is a proteasome subunit involved in the regulation of protein degradation. This protein has a molecular mass of approximately 34 kDa and forms an essential part of the 19S regulatory particle of the proteasome complex. PSMD14 is expressed in various tissues but shows significant expression levels in the brain heart and skeletal muscles. By deubiquitinating substrates PSMD14 contributes to the recycling of ubiquitin for further use in the cell’s ubiquitin-proteasome pathway.
Biological function summary

PSMD14 functions as a vital component of the 19S proteasome which is integrated into the proteolytic 26S proteasome complex. The protein's deubiquitinating activity plays a role in maintaining proteostasis by removing ubiquitin chains from substrates destined for degradation. This process is important for regulating protein quality control cell cycle progression and response to oxidative stress among other cellular processes. As part of the proteasome complex PSMD14’s activity impacts cellular homeostasis and contributes to cellular responses to various stimuli.

Pathways

PSMD14 is instrumental in the ubiquitin-proteasome system which is essential for targeted protein degradation modulation of the cell cycle and regulation of transcription factors. It interacts with other proteasome components like PSMD12 and PSMD3 to form a functional proteasome unit. This protein is significant in the NF-kB signaling pathway where it influences the degradation of inhibitor proteins allowing for the activation of NF-kB a critical transcription factor in immune response and cell survival.

PSMD14’s function connects it to cancer and neurodegenerative conditions. Deregulation or mutations in PSMD14 have been implicated in various cancers possibly through disturbed protein degradation which leads to aberrant cell cycle progression and survival. It also relates to neurodegenerative diseases like Alzheimer's as impaired proteasome function can lead to the accumulation of misfolded proteins. PSMD14 interacts with proteins like UCH37 which is also linked to the pathogenesis of these diseases highlighting the significance of controlled proteolysis in maintaining cellular health.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs) : acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.
See full target information PSMD14
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 19:55-63 PubMed36577875

2022

Targeted degradation via direct 26S proteasome recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Charlene Bashore,Sumit Prakash,Matthew C Johnson,Ryan J Conrad,Ivy A Kekessie,Suzie J Scales,Noriko Ishisoko,Tracy Kleinheinz,Peter S Liu,Nataliya Popovych,Aaron T Wecksler,Lijuan Zhou,Christine Tam,Inna Zilberleyb,Rajini Srinivasan,Robert A Blake,Aimin Song,Steven T Staben,Yingnan Zhang,David Arnott,Wayne J Fairbrother,Scott A Foster,Ingrid E Wertz,Claudio Ciferri,Erin C Dueber

Frontiers in endocrinology 12:757592 PubMed34975746

2021

Sperm Proteome Analysis to Investigate DNA Repair Mechanisms in Varicocele Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Renata Finelli,Sara Darbandi,Peter Natesan Pushparaj,Ralf Henkel,Edmund Ko,Ashok Agarwal

Molecular oncology 15:3639-3658 PubMed34382324

2021

Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshui Sun,Zhuonan Liu,Fangfang Bi,Qing Yang

Annals of translational medicine 9:1019 PubMed34277819

2021

The prognostic value of USP14 and PSMD14 expression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Lei,Xiaoliu Liu,Wei Liu,Yun Zhang,Zhihong Liu

Scientific reports 10:19704 PubMed33184426

2020

Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Sakai,Mayumi Yuasa-Sunagawa,Masayuki Kusakabe,Aiko Kishimoto,Takeshi Matsui,Yuki Kaneko,Jun-Ichi Akagi,Nicolas Huyghe,Masae Ikura,Tsuyoshi Ikura,Fumio Hanaoka,Masayuki Yokoi,Kaoru Sugasawa
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB109130

Anti-PSMD14 antibody [EPR4258]

primary-antibodies

psmd14-antibody-epr4258-ab109130

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(0 reviews)

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