Anti-PSMD1 抗体 (ab2941)
Key features and details
- Rabbit polyclonal to PSMD1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-PSMD1 antibody
PSMD1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PSMD1 -
由来種
Rabbit -
特異性
Detects proteasome Rpn2 from human cells. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey
交差が予測される動物種: Chicken -
免疫原
Synthetic peptide corresponding to Human PSMD1 aa 929-953.
Sequence:AAHGPKIEEEEQEPEPPEPFEYIDD
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2941の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa). By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract. |
ターゲット情報
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機能
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
配列類似性
Belongs to the proteasome subunit S1 family.
Contains 10 PC repeats. - Information by UniProt
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参照データベース
- Entrez Gene: 424926 Chicken
- Entrez Gene: 5707 Human
- Entrez Gene: 70247 Mouse
- Entrez Gene: 83806 Rat
- SwissProt: Q5F418 Chicken
- SwissProt: Q99460 Human
- SwissProt: Q3TXS7 Mouse
- SwissProt: O88761 Rat
see all -
別名
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
see all
画像
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All lanes : Anti-PSMD1 antibody (ab2941) at 1 µg/ml
Lane 1 : MCF7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : SK-OV-3 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : COS-7 cell lysate
Lane 7 : Jurkat cell lysate
Lane 8 : A431 cell lysate
Lane 9 : Mouse testis tissue lysate
Lane 10 : Rat testis tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab2941 は 11 報の論文で使用されています。
- Deshmukh FK et al. Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family. Nat Commun 14:3126 (2023). PubMed: 37253751
- Kim M et al. Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction. Nat Commun 14:4092 (2023). PubMed: 37433777
- Park HC et al. PSMD1 as a prognostic marker and potential target in oropharyngeal cancer. BMC Cancer 23:1242 (2023). PubMed: 38104103
- Dekel E et al. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun 12:1172 (2021). PubMed: 33608523
- Lu X et al. Structure-guided bifunctional molecules hit a DEUBAD-lacking hRpn13 species upregulated in multiple myeloma. Nat Commun 12:7318 (2021). PubMed: 34916494