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リコンビナントRabMAb

Anti-PSMC5 抗体 [EPR13566(B)] - C-terminal (ab180495)

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Western blot - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
  • Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
  • Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13566(B)] to PSMC5 - C-terminal
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PSMC5 antibody [EPR13566(B)] - C-terminal
    PSMC5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13566(B)] to PSMC5 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human PSMC5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P62195

  • ポジティブ･コントロール

    • HepG2, HeLa and 293T lysates; Human pancreas tissue; 293T and HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13566(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180495の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ICC/IF
1/500.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ICC/IF
1/500.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

  • 配列類似性

    Belongs to the AAA ATPase family.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 26S protease regulatory subunit 8 antibody
    • 26S proteasome AAA-ATPase subunit RPT6 antibody
    • Cim3 antibody
    • MSUG1 protein antibody
    • p45 antibody
    • p45/SUG antibody
    • Proteasome 26S ATPase subunit 5 antibody
    • Proteasome 26S subunit ATPase 5 antibody
    • Proteasome prosome macropain 26S subunit ATPase 5 antibody
    • Proteasome subunit p45 antibody
    • PRS8_HUMAN antibody
    • PSMC5 antibody
    • Rpt6 antibody
    • S8 antibody
    • SUG1 antibody
    • Tat binding protein homolog 10 antibody
    • TBP10 antibody
    • Thyroid hormone receptor interacting protein 1 antibody
    • Thyroid hormone receptor-interacting protein 1 antibody
    • Thyroid receptor interactor 1 antibody
    • TRIP1 antibody
    • TRIP1(SUG1) antibody
    see all

画像

  • Western blot - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
    Western blot - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
    All lanes : Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PSMC5 with ab180495 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
    Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

    Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)labeling PSMC5 with ab180495 at 1/30 dilution (green) compared to a negative control Rabbit monoclonal IgG (red) follwed byGoat anti rabbit IgG (FITC) secondary at 1/75 dilution. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
    Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

    Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/250 dilution (red) followed by with Goat anti rabbit IgG (Dylight 555) . DAPI staining (blue).

  • Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)
    Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

参考文献 (0)

ab180495 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180495 は論文での使用が確認できていません。

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