Anti-PSMB5/MB1 抗体 (ab3330)
Key features and details
- Rabbit polyclonal to PSMB5/MB1
- Suitable for: WB, ICC
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PSMB5/MB1 antibody
PSMB5/MB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PSMB5/MB1 -
由来種
Rabbit -
特異性
Detects proteasome PSMB5/MB1.
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アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Cow, Human -
免疫原
Synthetic peptide corresponding to Mouse PSMB5/MB1 aa 200 to the C-terminus.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3330の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
1/500 - 1/5000. Detects a band of approximately 22 kDa.
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ICC |
1/10 - 1/200.
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特記事項 |
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WB
1/500 - 1/5000. Detects a band of approximately 22 kDa. |
ICC
1/10 - 1/200. |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway. -
配列類似性
Belongs to the peptidase T1B family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 534640 Cow
- Entrez Gene: 5693 Human
- Entrez Gene: 19173 Mouse
- Omim: 600306 Human
- SwissProt: Q32KL2 Cow
- SwissProt: P28074 Human
- SwissProt: O55234 Mouse
- Unigene: 422990 Human
see all -
別名
- DKFZp459C139 antibody
- EC 3.4.25.1 antibody
- LMPX antibody
see all
画像
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All lanes : Anti-PSMB5/MB1 antibody (ab3330) at 1/1000 dilution
Lane 1 : NCI-H1299 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 3 : Mouse liver cell lysate
Lysates/proteins at 25 µg per lane.
Observed band size: 23 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of A431 (Human epidermoid carcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of BAEC (Bovine aortic endothelial cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of HeLa (Human epithelial adenocarcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (41)
ab3330 は 41 報の論文で使用されています。
- Farini A et al. Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice. EMBO Mol Med 15:e16244 (2023). PubMed: 36533294
- Waad Sadiq Z et al. Immunogenic cell death triggered by impaired deubiquitination in multiple myeloma relies on dysregulated type I interferon signaling. Front Immunol 14:982720 (2023). PubMed: 36936919
- Teixeira VON et al. The role of proteasome in muscle wasting of experimental arthritis. Adv Rheumatol 63:14 (2023). PubMed: 36949513
- Zhang C et al. ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma. J Cell Mol Med 26:5078-5094 (2022). PubMed: 36071546
- Wang Y et al. Tsc2 knockout counteracts ubiquitin-proteasome system insufficiency and delays photoreceptor loss in retinitis pigmentosa. Proc Natl Acad Sci U S A 119:e2118479119 (2022). PubMed: 35275792