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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PSMB10/MECL1 抗体 [EPR14902] (ab183506)

Submit a review Submit a question References (12)
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
  • Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
  • Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14902] to PSMB10/MECL1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-PSMB10/MECL1 antibody [EPR14902]
    PSMB10/MECL1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14902] to PSMB10/MECL1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, Daudi, HuT 78 and Raji cell lysates; Human fetal spleen and thymus tissue lysates. IHC-P: Human kidney adenocarcinoma tissue. Flow Cyt (intra): Daudi cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183506の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

  • 配列類似性

    Belongs to the peptidase T1B family.

  • 発生段階

    Highly expressed in immature dendritic cells (at protein level).

  • 翻訳後修飾

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • beta2i antibody
    • FLJ00366 antibody
    • LMP10 antibody
    • Low molecular mass protein 10 antibody
    • Macropain subunit MECl 1 antibody
    • Macropain subunit MECl-1 antibody
    • MECL1 antibody
    • MGC1665 antibody
    • Multicatalytic endopeptidase complex subunit MECl 1 antibody
    • Multicatalytic endopeptidase complex subunit MECl-1 antibody
    • OTTHUMP00000174858 antibody
    • Proteasome (prosome macropain) subunit beta type 10 antibody
    • Proteasome beta 10 subunit antibody
    • Proteasome catalytic subunit 2i antibody
    • Proteasome MECl 1 antibody
    • Proteasome MECl-1 antibody
    • Proteasome subunit beta 2i antibody
    • Proteasome subunit beta 7i antibody
    • Proteasome subunit beta type 10 antibody
    • Proteasome subunit beta type-10 antibody
    • Proteasome subunit beta-2i antibody
    • Proteasome subunit MECL1 antibody
    • PSB10_HUMAN antibody
    • PSMB 10 antibody
    • Psmb10 antibody
    see all

画像

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PSMB10 knockout HeLa cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab183506 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab183506 Anti-PSMB10/MECL1 antibody [EPR14902] was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used.  Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE.  ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

    Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

    Intracellular flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

     

     

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

    Lane 1 : Human fetal spleen tissue lysate
    Lane 2 : Human fetal thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 29 kDa

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution

    Lane 1 : HuT 78 cell line lysate
    Lane 2 : Raji cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 29 kDa

  • Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

参考文献 (12)

ab183506 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab183506 は 12 報の論文で使用されています。

  • Kim S  et al. Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates. Immune Netw 22:e28 (2022). PubMed: 35799704
  • Ng S  et al. STUB1 is an intracellular checkpoint for interferon gamma sensing. Sci Rep 12:14087 (2022). PubMed: 35982220
  • Liu EA  et al. TDP-43 proteinopathy occurs independently of autophagic substrate accumulation and underlies nuclear defects in Niemann-Pick C disease. Neuropathol Appl Neurobiol 47:1019-1032 (2021). PubMed: 34048071
  • Abusarah J  et al. Engineering immunoproteasome-expressing mesenchymal stromal cells: A potent cellular vaccine for lymphoma and melanoma in mice. Cell Rep Med 2:100455 (2021). PubMed: 35028603
  • Liao J  et al. Deficiency of LMP10 Attenuates Diet-Induced Atherosclerosis by Inhibiting Macrophage Polarization and Inflammation in Apolipoprotein E Deficient Mice. Front Cell Dev Biol 8:592048 (2020). PubMed: 33195259
View all Publications for this product

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