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AB109532

Anti-PSMA3 抗体 [EPR5455]

Anti-PSMA3 antibody [EPR5455]

4

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal PSMA3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

別名を表示する

HC8, PSC8, PSMA3, Proteasome subunit alpha type-3, Macropain subunit C8, Multicatalytic endopeptidase complex subunit C8, Proteasome component C8, Proteasome subunit alpha-7, alpha-7

3 Images
Western blot - Anti-PSMA3 antibody [EPR5455] (AB109532)
  • WB

Unknown

Western blot - Anti-PSMA3 antibody [EPR5455] (AB109532)

All lanes:

Western blot - Anti-PSMA3 antibody [EPR5455] (ab109532) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Predicted band size: 28 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-PSMA3 antibody [EPR5455] (AB109532)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMA3 antibody [EPR5455] (AB109532)

ab109532 at 1/100 dilution staining PSMA3 in HeLa cells by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA3 antibody [EPR5455] (AB109532)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA3 antibody [EPR5455] (AB109532)

ab109532 at 1/100 dilution staining PSMA3 in paraffin embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-PSMA3 antibody [EPR5455] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5455

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
See full target information PSMA3
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 52:338-351 PubMed34755333

2021

Immuno-oncological role of 20S proteasome alpha-subunit 3 in aggravating the progression of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyan Liu,Jingwen Shao,Chaoqi Zhang,Guohui Qin,Jiayin Liu,Miaomiao Li,Peng Wu,Xuan Zhao,Yi Zhang

Veterinary immunology and immunopathology 226:110069 PubMed32535163

2020

The IFN-γ-induced immunoproteasome is suppressed in highly pathogenic porcine reproductive and respiratory syndrome virus-infected alveolar macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Liu,Yue-Yang Yu,Huai-Yu Wang,Jing-Fei Wang,Xi-Jun He

iScience 9:433-450 PubMed30469013

2018

USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiko Takahashi,Hiroki Kitaura,Akiyoshi Kakita,Taichi Kakihana,Yoshinori Katsuragi,Masaaki Nameta,Lu Zhang,Yuriko Iwakura,Hiroyuki Nawa,Masaya Higuchi,Masaaki Komatsu,Masahiro Fujii

Scientific reports 7:9365 PubMed28839214

2017

The role of the immunoproteasome in interferon-γ-mediated microglial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kasey E Moritz,Nikki M McCormack,Mahlet B Abera,Coralie Viollet,Young J Yauger,Gauthaman Sukumar,Clifton L Dalgard,Barrington G Burnett

Cancer cell international 17:42 PubMed28373828

2017

The synthetic peptide CIGB-300 modulates CK2-dependent signaling pathways affecting the survival and chemoresistance of non-small cell lung cancer cell lines.

Applications

ICC/IF

Species

Human

Stéfano M Cirigliano,María I Díaz Bessone,Damián E Berardi,Carolina Flumian,Elisa D Bal de Kier Joffé,Silvio E Perea,Hernán G Farina,Laura B Todaro,Alejandro J Urtreger

Scientific reports 7:40698 PubMed28079188

2017

Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths.

Applications

WB

Species

Chicken

Yongtao Li,Fan Ming,Huimin Huang,Kelei Guo,Huanchun Chen,Meilin Jin,Hongbo Zhou

The Journal of biological chemistry 290:20661-73 PubMed26070558

2015

Protein Interaction between Ameloblastin and Proteasome Subunit α Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhui Geng,Shane N White,Michael L Paine,Malcolm L Snead
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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