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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PSIP1/LEDGF 抗体 [EPR11890] (ab177159)

Submit a review Submit a question References (1)
Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11890] to PSIP1/LEDGF
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PSIP1/LEDGF antibody [EPR11890]
    PSIP1/LEDGF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11890] to PSIP1/LEDGF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human PSIP1/LEDGF aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75475

  • ポジティブ･コントロール

    • WB: HEK293T, Jurkat, HeLa and K562 cell lysates. IHC-P: Human brain and hepatocellular carcinoma tissues. ICC/IF: K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177159の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.

  • 組織特異性

    Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.

  • 関連疾患

    Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.

  • 配列類似性

    Belongs to the HDGF family.
    Contains 1 PWWP domain.

  • ドメイン

    Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus. Remains chromatin-associated throughout the cell cycle.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CLL associated antigen KW 7 antibody
      • CLL-associated antigen KW-7 antibody
      • Dense fine speckles 70 kDa protein antibody
      • DFS 70 antibody
      • DFS70 antibody
      • LEDGF antibody
      • Lens epithelium derived growth factor antibody
      • Lens epithelium-derived growth factor antibody
      • MGC74712 antibody
      • p52 antibody
      • p75 antibody
      • PAIP antibody
      • PC4 and SFRS1 interacting protein 1 antibody
      • PC4 and SFRS1 interacting protein 2 antibody
      • PC4 and SFRS1 interacting protein antibody
      • PC4 and SFRS1-interacting protein antibody
      • PSIP 1 antibody
      • PSIP 2 antibody
      • Psip1 antibody
      • PSIP1_HUMAN antibody
      • PSIP2 antibody
      • Transcriptional coactivator p75/p52 antibody
      see all

    画像

    • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : PSIP1 knockout HEK293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 60 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab177159 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab177159 Anti-PSIP1/LEDGF antibody [EPR11890] was shown to specifically react with PSIP1/LEDGF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266464 (knockout cell lysate ab258144) was used. Wild-type and PSIP1/LEDGF knockout samples were subjected to SDS-PAGE. ab177159 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

      Immunofluorescent analysis of K562 cells labeling PSIP1/LEDGF with ab177159 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 60 kDa

    • Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

    参考文献 (1)

    ab177159 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab177159 は 1 報の論文で使用されています。

    • Li L & Wang Y Cross-talk between the H3K36me3 and H4K16ac histone epigenetic marks in DNA double-strand break repair. J Biol Chem 292:11951-11959 (2017). PubMed: 28546430

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