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Anti-PSGL-1 抗体 [HECA-452] (ab110096)

Reviews (1)Q&A (1)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)

Key features and details

  • Rat monoclonal [HECA-452] to PSGL-1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgM

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PSGL-1 antibody [EPR22504-36] (ab227836)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PSGL-1 antibody [HECA-452]
    PSGL-1 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [HECA-452] to PSGL-1

  • 由来種

    Rat

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Tissue, cells or virus corresponding to Human PSGL-1. Stromal preparation of human tonsils.

  • ポジティブ･コントロール

    • Human spleen or tonsil tissue. Human peripheral blood lymphocytes.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab110096の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 10 µg/ml.
特記事項
IHC-P
Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能

    A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.

  • 組織特異性

    Expressed on neutrophils, monocytes and most lymphocytes.

  • 翻訳後修飾

    Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 162 antibody
      • CD162 antibody
      • CD162 antigen antibody
      • CLA antibody
      • Cutaneous lymphocyte associated associated antigen antibody
      • P selectin glycoprotein ligand 1 antibody
      • P selectin glycoprotein ligand 1 precursor antibody
      • P-selectin glycoprotein ligand 1 antibody
      • PSGL 1 antibody
      • PSGL-1 antibody
      • PSGL1 antibody
      • Selectin P ligand antibody
      • SELPL_HUMAN antibody
      • SELPLG antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)

      ab110096 at 10µg/ml staining PSGL-1 in Formalin/PFA-fixed paraffin-embedded Human spleen tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)

      ab110096 at 10µg/ml staining PSGL-1 in Formalin/PFA-fixed paraffin-embedded Human tonsil tissue.

    参考文献 (3)

    ab110096 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab110096 は 3 報の論文で使用されています。

    • Granai M  et al. Impact of P-selectin-PSGL-1 Axis on Platelet-Endothelium-Leukocyte Interactions in Fatal COVID-19. Lab Invest 103:100179 (2023). PubMed: 37224922
    • Arnold S  et al. Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues. Methods Protoc 5:N/A (2022). PubMed: 36136817
    • Almaraz RT  et al. Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Mol Cell Proteomics 11:M112.017558 (2012). PubMed: 22457533

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD162 antibody [HECA-452]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (LPS-inflamed lung)
    Permeabilization
    No
    Specification
    LPS-inflamed lung
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 07 2016

    Question

    I would like to test this antibody in human cells in immunocytochemistry (ICC/IF), an untested application.

    Read More
    Answer

    I am very pleased to hear you would like to accept our offer and test ab110096 in ICC/IF. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com