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リコンビナントRabMAb

Anti-PSF1 抗体 [EPR13359] (ab181112)

Submit a review Submit a question References (6)
Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Anti-PSF1 antibody [EPR13359] (ab181112)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13359] to PSF1
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PSF1 antibody [EPR13359]
    PSF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13359] to PSF1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (intra): HepG2 cells ICC/IF: HeLa and HepG2 cells WB: Jurkat, HeLa and HepG2 whole cell lysate (ab7900); human fetal heart tissue lysate, mouse and rat heart tissue lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181112の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF
1/100.

For unpurified format use at 1/250 - 1/500 dilution.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF
1/100.

For unpurified format use at 1/250 - 1/500 dilution.
Flow Cyt (Intra)
Use at an assay dependent concentration.
追加情報
Is unsuitable for IP.

ターゲット情報

画像

  • Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
    Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
    All lanes : Anti-PSF1 antibody [EPR13359] (ab181112) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human fetal heart lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (ab181112)
    Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (ab181112)

    Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/70 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
    Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/100 dilution (7.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
    Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
    All lanes : Anti-PSF1 antibody [EPR13359] (ab181112) at 1/20000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
    Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with ab181112 (unpurified) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Anti-PSF1 antibody [EPR13359] (ab181112)
    Anti-PSF1 antibody [EPR13359] (ab181112)

参考文献 (6)

ab181112 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181112 は 6 報の論文で使用されています。

  • Li S  et al. GINS1 Induced Sorafenib Resistance by Promoting Cancer Stem Properties in Human Hepatocellular Cancer Cells. Front Cell Dev Biol 9:711894 (2021). PubMed: 34414190
  • Zhang J  et al. DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. Nat Commun 11:3951 (2020). PubMed: 32769987
  • Bu F  et al. Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients. Onco Targets Ther 13:11433-11444 (2020). PubMed: 33192076
  • Coulombe P  et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun 10:2426 (2019). PubMed: 31160578
  • Huang J  et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
  • Li X  et al. Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72:650-660.e8 (2018). PubMed: 30392930

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