Anti-PSD95 抗体 [7E3-1B8] - Synaptic Marker (ab13552)
Key features and details
- Mouse monoclonal [7E3-1B8] to PSD95 - Synaptic Marker
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker
PSD95 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7E3-1B8] to PSD95 - Synaptic Marker -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Recombinant full length protein corresponding to Rat PSD95.
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ポジティブ・コントロール
- ICC/IF: Rat primary hippocampal neurons; HaCaT cells; mouse backskin tissue section. WB: Rat brain membrane lysate; bovine brain tissue extract. Flow Cyt: K562 cells. IHC-P: Mouse backskin tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
7E3-1B8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13552の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. -
組織特異性
Brain. -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
ドメイン
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
翻訳後修飾
Palmitoylation of isoform 1 is required for targeting to postsynaptic density. -
細胞内局在
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. - Information by UniProt
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参照データベース
- Entrez Gene: 100137840 Cow
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
see all -
別名
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
画像
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Central Panel: ab13552 staining PSD95 in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
Left-hand panel: Homer GFP.
Right-hand panel: Merge. -
Western blot analysis of bovine brain tissue extract, probed with ab13552. Western blot analysis of bovine brain tissue extract, probed with ab13552.
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Overlay histogram showing K562 cells stained with ab13552 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13552, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.
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Immunocytochemistry/ Immunofluorescence analysis of HaCaT cells labeling PSD95 with ab13552 at 1/100 dilution. Cells were fixed with 00% cold methanol for 10 minutes at -20°C. A FITC Goat Anti-Mouse antibody at 1/50 was used as secondary. The image shows Filamentouslike staining.
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Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552) at 1/1000 dilution + Rat brain membrane lysate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab13552 は 54 報の論文で使用されています。
- Jia M et al. Liraglutide ameliorates delirium-like behaviors of aged mice undergoing cardiac surgery by mitigating microglia activation via promoting mitophagy. Psychopharmacology (Berl) 241:687-698 (2024). PubMed: 37968531
- Fracassi A et al. TREM2-induced activation of microglia contributes to synaptic integrity in cognitively intact aged individuals with Alzheimer's neuropathology. Brain Pathol 33:e13108 (2023). PubMed: 35816404
- Cervetto C et al. Assessment of neurotransmitter release in human iPSC-derived neuronal/glial cells: a missing in vitro assay for regulatory developmental neurotoxicity testing. Reprod Toxicol 117:108358 (2023). PubMed: 36863571
- Hassan Z et al. Cognitive Impairment Is Associated with AMPAR Glutamatergic Dysfunction in a Mouse Model of Neuronal Methionine Synthase Deficiency. Cells 12:N/A (2023). PubMed: 37174668
- Qi F et al. VEGF-A in serum protects against memory impairment in APP/PS1 transgenic mice by blocking neutrophil infiltration. Mol Psychiatry 28:4374-4389 (2023). PubMed: 37280283