Anti-PRSS35 抗体 (ab151970)
Key features and details
- Rabbit polyclonal to PRSS35
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRSS35 antibody -
製品の詳細
Rabbit polyclonal to PRSS35 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-243 of Human PRSS35 (Q8N3Z0).
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ポジティブ･コントロール
- PC3, SK N SH and Mouse testis whole cell lysates; Human U251 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151970の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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関連性
PRSS35, although related to the peptidase S1 family, lacks the conserved active Ser residue in position 346 which is replaced by a Thr, suggesting that it has no protease activity. This Ser to Thr substitution may play an important role in determining a unique pattern of substrate specificity for PRSS35. Alternatively, this substitution may yield an inactive protease that binds to specific substrates, thus protecting them from proteolysis. The Mouse Prss35 gene is selectively expressed in the ovary and it's expression prior to ovulation is, in part, regulated by progesterone. PRSS35 may be involved in ovulation and the formation and regression of the corpus luteum. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 167681 Human
- Entrez Gene: 244954 Mouse
- SwissProt: Q8N3Z0 Human
- SwissProt: Q8C0F9 Mouse
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別名
- 6030424L22Rik antibody
- C6orf158 antibody
- Inactive serine protease 35 antibody
see all
画像
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Western blot - Anti-PRSS35 antibody (ab151970)Anti-PRSS35 antibody (ab151970) at 1/1000 dilution + Mouse testis whole cell lysate at 50 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Western blot - Anti-PRSS35 antibody (ab151970)All lanes : Anti-PRSS35 antibody (ab151970) at 1/1000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : SK N SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS35 antibody (ab151970)Immunohistochemical analysis of paraffin-embedded Human U251 xenograft tissue labeling PRSS35 with ab151970 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab151970 は 2 報の論文で使用されています。
- Li Y et al. Silencing HE4 alleviates the renal fibrosis in lupus nephritis mice by regulating the C3/MMPs/prss axis. Naunyn Schmiedebergs Arch Pharmacol 397:4823-4831 (2024). PubMed: 38157023
- Van Hove L et al. Fibrotic enzymes modulate wound-induced skin tumorigenesis. EMBO Rep 22:e51573 (2021). PubMed: 33780134