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リコンビナントRabMAb

Anti-PRSS2 抗体 [EPR25648-5] (BSA and Azide free) (ab302604)

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Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25648-5] to PRSS2 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free)
    PRSS2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25648-5] to PRSS2 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human pancreas tissue lysate, His-tagged PRSS2 recombinant protein, IHC-P: Human pancreas tissue.

  • 特記事項

    ab302604 is a carrier free version of ab302603. 

    ab302603 does not react in: WB and IHC-P with mouse and rat species; ICC, intracellular flow cytometrey, and immunoprecipitation with human tissues. 

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25648-5

  • アイソタイプ

    IgG

  • 研究分野

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302604の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 25-30 kDa (predicted molecular weight: 26 kDa).
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 25-30 kDa (predicted molecular weight: 26 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

ターゲット情報

  • 配列類似性

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.

  • 翻訳後修飾

    Sulfated on tyrosine.

  • 細胞内局在

    Secreted > extracellular space.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Anionic trypsinogen antibody
      • EC 3.4.21.4 antibody
      • MGC111183 antibody
      • MGC120174 antibody
      • Protease serine 2 preproprotein antibody
      • Protease, serine, 2 (trypsin 2) antibody
      • Protease, serine, 2 antibody
      • Protease, serine, 2, preproprotein antibody
      • Prss2 antibody
      • Serine protease 2 antibody
      • TRY2 antibody
      • TRY2_HUMAN antibody
      • TRY8 antibody
      • TRYP2 antibody
      • Trypsin 2 antibody
      • Trypsin II antibody
      • Trypsin-2 antibody
      • Trypsinogen 2 antibody
      see all

    画像

    • Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      All lanes : Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1000 cells

      Lane 1 : Human pancreas tissue lysate
      Lane 2 : Human kidney tissue lysate
      Lane 3 : Human testis tissue lysate

      Lysates/proteins at 5 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      This data was developed using ab302603, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Negative controls: kidney, testis (PMID: 9736042). 

      Exposure time: 3.25 seconds. 

    • Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      All lanes : Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution

      Lane 1 : His-tagged PRSS1 recombinant protein, 10 ng
      Lane 2 : His-tagged PRSS2 recombinant protein, 10 ng
      Lane 3 : His-tagged PRSS3 recombinant protein, 10 ng
      Lane 4 : His-tagged PRSS3P2 recombinant protein, 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 26 kDa
      Observed band size: 25-30 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab302603, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      This antibody does not cross-react with human PRSS1, PRSS3 and PRSS3P2.

    • Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell fresh lysate, 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab302603, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The identity of the band at approximately 80 kDa is unknown.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

      This data was developed using ab302603, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PRSS2 with ab302603 at 1/2000 dilution (0.256 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Postitive staining on acinar cells of human pancreas. The section was incubated with ab302603 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

      This data was developed using ab302603, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling PRSS2 with ab302603 at 1/2000 dilution (0.256 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on human kidney. The section was incubated with ab302603 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
      Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302604 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302604 は論文での使用が確認できていません。

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