Anti-PRPF8/Prp8 抗体 (ab245504)
Key features and details
- Rabbit polyclonal to PRPF8/Prp8
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRPF8/Prp8 antibody
PRPF8/Prp8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PRPF8/Prp8 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human PRPF8/Prp8 aa 1825-1875. The exact sequence is proprietary. (NP_006436.3).
Database link: Q6P2Q9 -
ポジティブ･コントロール
- WB: HEK-293T, HeLa, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245504 was affinity purified using an epitope specific to PRPF8/Prp8 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245504の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 274 kDa.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 274 kDa. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes. -
組織特異性
Widely expressed. -
関連疾患
Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant. -
配列類似性
Contains 1 MPN (JAB/Mov34) domain. -
ドメイン
The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus speckle. - Information by UniProt
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参照データベース
- Entrez Gene: 10594 Human
- Entrez Gene: 192159 Mouse
- Omim: 607300 Human
- SwissProt: Q6P2Q9 Human
- SwissProt: Q99PV0 Mouse
- Unigene: 181368 Human
- Unigene: 3757 Mouse
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別名
- 220 kDa U5 snRNP specific protein antibody
- 220 kDa U5 snRNP-specific protein antibody
- Apoptosis regulated protein 1 antibody
see all
画像
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Western blot - Anti-PRPF8/Prp8 antibody (ab245504)All lanes : Anti-PRPF8/Prp8 antibody (ab245504) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 274 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-PRPF8/Prp8 antibody (ab245504)PRPF8/Prp8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245504 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245504 at 1 µg/ml.
Lane 1: ab245504 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody (ab245504)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRPF8/Prp8 using ab245504 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245504 は論文での使用が確認できていません。