Anti-PRP16 抗体 [EPR9845] (ab154801)
![Western blot - Anti-PRP16 antibody [EPR9845] (ab154801)](https://www.abcam.co.jp/ps/products/154/ab154801/Images/DHX38-Primary-antibodies-ab154801-2.jpg "Western blot - Anti-PRP16 antibody [EPR9845] (ab154801)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9845] to PRP16
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PRP16 antibody [EPR9845]
PRP16 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9845] to PRP16 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human PRP16. The exact sequence is proprietary.
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ポジティブ･コントロール
- Raji, Jurkat, K562, 293T and HeLa cell lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9845 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 141 kDa.
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| ICC/IF |
1/100 - 1/250.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 141 kDa. |
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ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.
ターゲット情報
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機能
Probable ATP-binding RNA helicase involved in pre-mRNA splicing. -
配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9785 Human
- GenBank: NM_014003 Human
- Omim: 605584 Human
- SwissProt: Q92620 Human
- Unigene: 151218 Human
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別名
- ATP dependent RNA helicase DHX38 antibody
- ATP-dependent RNA helicase DHX38 antibody
- DDX38 antibody
see all
画像
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Western blot - Anti-PRP16 antibody [EPR9845] (ab154801)All lanes : Anti-PRP16 antibody [EPR9845] (ab154801) at 1/1000 dilution
Lane 1 : Raji lysate
Lane 2 : Jurkat lysate
Lane 3 : K562 lysate
Lane 4 : 293T lysate
Lane 5 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 141 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PRP16 antibody [EPR9845] (ab154801)](https://www.abcam.co.jp/ps/products/154/ab154801/Images/DHX38-Primary-antibodies-ab154801-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRP16 antibody [EPR9845] (ab154801)Immunofluorescence analysis of HeLa cells labeling PRP16 with ab154801 at 1/100 dilution.
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![Anti-PRP16 antibody [EPR9845] (ab154801)](https://www.abcam.co.jp/ps/products/154/ab154801/Images/ab154801-3-benefits-of-recombinant-antibodies.png)
Anti-PRP16 antibody [EPR9845] (ab154801)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154801 は 1 報の論文で使用されています。
- Shakyawar DK et al. C3G dynamically associates with nuclear speckles and regulates mRNA splicing. Mol Biol Cell 29:1111-1124 (2018). PubMed: 29496966