Anti-PROX1 抗体 (ab38692)
Key features and details
- Rabbit polyclonal to PROX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PROX1 antibody
PROX1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PROX1 -
由来種
Rabbit -
特異性
We have had mixed results for use of this antibody in IHC-P. The AbReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. We welcome any feedback from customers who have used this antibody in IHC-P.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human PROX1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38691) -
ポジティブ･コントロール
- WB: HepG2, SHSY5Y whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38692の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa). |
ターゲット情報
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機能
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. -
組織特異性
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the Prospero homeobox family.
Contains 1 Prospero-type homeobox DNA-binding domain. -
ドメイン
The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity. -
細胞内局在
Nucleus. RORG promotes its nuclear localization. - Information by UniProt
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参照データベース
- Entrez Gene: 5629 Human
- Entrez Gene: 19130 Mouse
- Entrez Gene: 305066 Rat
- Omim: 601546 Human
- SwissProt: Q92786 Human
- SwissProt: P48437 Mouse
- Unigene: 741808 Human
- Unigene: 744931 Human
see all -
製品の状態
Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter. -
別名
- Homeobox prospero like protein antibody
- Homeobox prospero like protein PROX1 antibody
- Homeobox prospero-like protein PROX1 antibody
see all
画像
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Western blot - Anti-PROX1 antibody (ab38692)All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml
Lane 1 : HepG2 whole cell lysate
Lane 2 : SHSY5Y whole cell lysate
Lane 3 : Hek293 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesGel type: MOPS
Blocking buffer: 3% milk
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab38692 は 15 報の論文で使用されています。
- Jo M et al. Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping. Microcirculation 30:e12788 (2023). PubMed: 36169611
- Genescu I et al. Dynamic interplay between thalamic activity and Cajal-Retzius cells regulates the wiring of cortical layer 1. Cell Rep 39:110667 (2022). PubMed: 35417707
- Koike H et al. Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells. Nat Protoc 16:919-936 (2021). PubMed: 33432231
- Taha E et al. eEF2/eEF2K Pathway in the Mature Dentate Gyrus Determines Neurogenesis Level and Cognition. Curr Biol 30:3507-3521.e7 (2020). PubMed: 32707059
- Shibata-Germanos S et al. Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges. Acta Neuropathol 139:383-401 (2020). PubMed: 31696318