Anti-PROX1 抗体 [5G10] (ab33219)
![Western blot - Anti-PROX1 antibody [5G10] (ab33219)](https://www.abcam.co.jp/ps/products/33/ab33219/Images/ab33219-46513-anti-prox1-antibody-5g10-western-blot.jpg "Western blot - Anti-PROX1 antibody [5G10] (ab33219)")
Key features and details
- Mouse monoclonal [5G10] to PROX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PROX1 antibody [5G10]
PROX1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5G10] to PROX1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive result in HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate .
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
5G10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33219の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 10 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 10 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. -
組織特異性
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the Prospero homeobox family.
Contains 1 Prospero-type homeobox DNA-binding domain. -
ドメイン
The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity. -
細胞内局在
Nucleus. RORG promotes its nuclear localization. - Information by UniProt
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参照データベース
- Entrez Gene: 5629 Human
- Entrez Gene: 305066 Rat
- Omim: 601546 Human
- SwissProt: Q92786 Human
- Unigene: 741808 Human
- Unigene: 744931 Human
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製品の状態
Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter. -
別名
- Homeobox prospero like protein antibody
- Homeobox prospero like protein PROX1 antibody
- Homeobox prospero-like protein PROX1 antibody
see all
画像
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Western blot - Anti-PROX1 antibody [5G10] (ab33219)All lanes : Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml
Lane 1 :Hep G2 nuclear extract lysate (ab14660)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 20 minutes -
![Western blot - Anti-PROX1 antibody [5G10] (ab33219)](https://www.abcam.co.jp/ps/products/33/ab33219/Images/ab33219-46511-anti-prox1-antibody-5g10-western-blot.jpg)
Western blot - Anti-PROX1 antibody [5G10] (ab33219)Image courtesy of an Abreview submitted by Dr. Xia ChenAnti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml + P3 Dentate gyrus whole tissue lysate at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab33219 は 6 報の論文で使用されています。
- Chutipongpisit K et al. Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer. Int J Exp Pathol 102:268-278 (2021). PubMed: 34791715
- Duan Z et al. Nuclear localization of Newcastle disease virus matrix protein promotes virus replication by affecting viral RNA synthesis and transcription and inhibiting host cell transcription. Vet Res 50:22 (2019). PubMed: 30894203
- Thestrup MI et al. A morphogenetic EphB/EphrinB code controls hepatopancreatic duct formation. Nat Commun 10:5220 (2019). PubMed: 31745086
- Lara NLM et al. Dibutyl phthalate induced testicular dysgenesis originates after seminiferous cord formation in rats. Sci Rep 7:2521 (2017). IF ; Rat . PubMed: 28566680
- Blancas AA et al. Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium. Cells Tissues Organs 201:268-76 (2016). PubMed: 27144771
- Chen X et al. Production of monoclonal antibodies against Prox1. Hybridoma (Larchmt) 25:27-33 (2006). PubMed: 16475879