Anti-Protor-1 抗体 - C-terminal (ab185995)
Key features and details
- Rabbit polyclonal to Protor-1 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Protor-1 antibody - C-terminal
Protor-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Protor-1 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee
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免疫原
Synthetic peptide within Human Protor-1 aa 300 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P85299 -
ポジティブ･コントロール
- 293T, HeLa, and Jurkat whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185995の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/20000 - 1/100000. Predicted molecular weight: 43 kDa.
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| 特記事項 |
|---|
|
WB
1/20000 - 1/100000. Predicted molecular weight: 43 kDa. |
ターゲット情報
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機能
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer. -
組織特異性
Most abundant in kidney and liver. Also highly expressed in brain, spleen, testis and placenta. Overexpressed in several colorectal tumors. -
配列類似性
Belongs to the PROTOR family. - Information by UniProt
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参照データベース
- Entrez Gene: 100612312 Chimpanzee
- Entrez Gene: 55615 Human
- Omim: 609406 Human
- SwissProt: P85299 Human
- Unigene: 102336 Human
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別名
- Arhgap8 antibody
- AU043908 antibody
- C030017C09Rik antibody
see all
画像
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Western blot - Anti-Protor-1 antibody - C-terminal (ab185995)All lanes : Anti-Protor-1 antibody - C-terminal (ab185995) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 3 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185995 は論文での使用が確認できていません。