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Anti-Protocadherin Gamma (pan) 抗体 [N159/5] - C-terminal (ab187186)

Submit a review Submit a question References (1)
Western blot - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)
  • Immunocytochemistry/ Immunofluorescence - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)

Key features and details

  • Mouse monoclonal [N159/5] to Protocadherin Gamma (pan) - C-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal

  • 製品の詳細

    Mouse monoclonal [N159/5] to Protocadherin Gamma (pan) - C-terminal

  • 由来種

    Mouse

  • 特異性

    Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins.

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Recombinant fragment within Mouse Protocadherin Gamma (pan) aa 800 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. The immunogens sequence is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789- 912 and C subfamily amino acids ~818-941).
    Database link: NP_291062.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Rat brain lysate.

  • 特記事項

    The clone number has been updated from S159-5 to N159/5, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    N159/5

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187186の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB
1/1000.
特記事項
ICC/IF
1/100.
WB
1/1000.

画像

  • Western blot - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)
    Western blot - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)
    Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186) at 1/1000 dilution + Rat brain membrane lysate at 10 µg

    Secondary
    Goat Anti-Mouse HRP at 1/200 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)
    Immunocytochemistry/ Immunofluorescence - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)

    Ab187186 staining Protocadherin gamma (pan) in SK-N-BE cells by Immunocytochemistry/Immunofluorescence (ICC/IF).  Cells were fixed with 4% formaldehyde for 15 minutes at room temperature. Samples were incubated wth pirmary antibody at 1/100 dilution for 60 minutes at room temperature. Phalliodin Texas Red F-Actin (1/1000) was used as a counterstain at 1/1000 dilution. DAPI nuclear counterstain was used at 1/5000 dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab187186 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187186 は 1 報の論文で使用されています。

  • Kaur G  et al. Alternative splicing of helicase-like transcription factor (Hltf): Intron retention-dependent activation of immune tolerance at the feto-maternal interface. PLoS One 13:e0200211 (2018). PubMed: 29975766

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