Name: Anti-Protein CASP antibody [2A10] - BSA and Azide free
SKU: ab54583
Rating: 4 (9 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

Key features and details

Mouse monoclonal [2A10] to Protein CASP - BSA and Azide free
Suitable for: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cyt
Reacts with: Human, Recombinant fragment
Isotype: IgG1

製品名

Anti-Protein CASP antibody [2A10] - BSA and Azide free
Protein CASP 一次抗体製品一覧
製品の詳細

Mouse monoclonal [2A10] to Protein CASP - BSA and Azide free
由来種

Mouse
アプリケーション

適用あり: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cytmore details
種交差性

交差種: Human, Recombinant fragment
免疫原

Recombinant fragment (GST-tag) corresponding to Human Protein CASP aa 500-650. NP_001904.2
Database link: Q13948

Run BLAST with Run BLAST with
ポジティブ･コントロール
- ICC/IF: HeLa cells. IHC-P: Human spleen tissue; human malignant lymphoma, diffuse large B tissue. Flow Cytometry: MCF7 cells. WB: Protein CASP transfected HEK-293T cell lysate. Recombinant Protein CASP protein.
特記事項

This product was changed from ascites to tissue culture supernatant on 13^th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファー

pH: 7.40
Constituent: PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Tissue culture supernatant
ポリ/モノ

モノクローナル
クローン名

2A10
アイソタイプ

IgG1
軽鎖の種類

kappa
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab54583の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P	(1)	Use at an assay dependent concentration.
WB	(1)	Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa.
ICC/IF	(3)	Use at an assay dependent concentration.
Sandwich ELISA		Use at an assay dependent concentration.
Flow Cyt		Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa.
ICC/IF Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

機能

May be involved in intra-Golgi retrograde transport.
配列類似性

Belongs to the CASP family.
細胞内局在

Golgi apparatus membrane.
Target information above from: UniProt accession Q13948 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 1523 Human
- SwissProt: Q13948 Human
- Unigene: 191482 Human
別名
- CASP_HUMAN antibody
- CUX1 antibody
- Protein CASP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.

This image was generated using the ascites version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein CASP (green) using ab54583 at 10 µg/ml in ICC/IF.

This image was generated using the ascites version of the product.
Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 µg/1 x 10⁶ cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1 x 10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.
Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

All lanes : Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml

Lane 1 : Protein CASP transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 77.5 kDa

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.

This image was generated using the ascites version of the product.
Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml + Tagged recombinant Protein CASP protein

Predicted band size: 77.5 kDa

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Expected MW 38 kDa.

This image was generated using the ascites version of the product.
Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)

Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody.

This image was generated using the ascites version of the product.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

ab54583 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab54583 は 24 報の論文で使用されています。

Kettunen P et al. SARS-CoV-2 Infection of Human Neurons Is TMPRSS2 Independent, Requires Endosomal Cell Entry, and Can Be Blocked by Inhibitors of Host Phosphoinositol-5 Kinase. J Virol 97:e0014423 (2023). PubMed: 37039676
Fame RM et al. Age-appropriate potassium clearance from perinatal cerebrospinal fluid depends on choroid plexus NKCC1. Fluids Barriers CNS 20:45 (2023). PubMed: 37328833
Papes F et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun 13:2387 (2022). PubMed: 35501322
Schilders KAA et al. Identification of SOX2 Interacting Proteins in the Developing Mouse Lung With Potential Implications for Congenital Diaphragmatic Hernia. Front Pediatr 10:881287 (2022). PubMed: 35615634
Rosebrock D et al. Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol 24:981-995 (2022). PubMed: 35697781

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 10 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (iPSC-derived cortical neurons DIV90)

Permeabilization

Yes - 0.3% Triton X-100

Specification

iPSC-derived cortical neurons DIV90

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 06 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (cortex)

Permeabilization

Yes - 3% Triton x100

Specification

cortex

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 26 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (cortical neurons)

Permeabilization

Yes - 0.25% Triton X-100 in PBS

Specification

cortical neurons

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 06 2018

Application

Immunohistochemistry free floating

Sample

Mouse Tissue sections (P12 brain sections)

Specification

P12 brain sections

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Bhavin Shah

Verified customer

投稿 Apr 17 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Rabbit Tissue sections (brain)

Permeabilization

Yes - 0.3% triton

Specification

brain

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 28 2017

Application

Immunocytochemistry

Sample

Human Cultured Cells (HeLa)

Permeabilization

Yes - 0.3% Triton-X-100 in PBS

Specification

HeLa

Blocking step

10% normal serum, 1% BSA in PBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 02 2013

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Rat Tissue sections (Brain)

Antigen retrieval step

None

Permeabilization

Specification

Brain

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sophie Pezet

Verified customer

投稿 Jun 08 2012

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (brain, placenta)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C

Permeabilization

Specification

brain, placenta

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 09 2011

Application

Western blot

Sample

Human Cell lysate - whole cell (MDA-MB-231, HT-1080, NIH3T3, D283Med, Daoy)

Gel Running Conditions

Reduced Denaturing (8%gel)

Loading amount

500000 cells

Specification

MDA-MB-231, HT-1080, NIH3T3, D283Med, Daoy

Blocking step

3% non fat milk/ 2% BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Sabine Topka

Verified customer

投稿 Oct 07 2009

Question

Dear Sir or Madam, I am interested in Anti-CUTL1 antibody and have some questions about it. There are 5 types anti-CUTL1 antibodies in the webpage. Anti-CUTL1 antibody (ab73885) looks better according to WB. What is the antibody's charaterization? Is it manufactured in-house or from outside labs? According to the reference, one of the antibodies is from xxxxxxxx group. Is ab73885 the right one? Many Thanks.

Abcam community

Verified customer

Asked on Oct 18 2011

Answer

Thank you for contacting us and sorry for the delay in getting back to you. We have a number of collaborators which produce antibodies for us as well as in house production of antibodies. The 5 antibodies that we have against CUTL1 have all been sourced through collaborators. All of the antibodies have been characterised through Western blotting and ab54583 and ab11584 has also been used in IHC. The diffferences observed in the western blots may be due to the antibodies being produced against different isoforms of CULT1. Whilst ab73885, ab85994 and ab98273 have been raised against immunogen sequences from CULT1 isoform 1 (SwissProt P39880) and would be expected to react with all CULT1 isoforms, ab115854 and ab54583 have both been raised against an immunogen sequence taken from CULT1 isoform 4 (SwissProt Q13948). These would therefore be expected to recognise isoform 4 and isoform 8 of CULT1 only (expected at around 77 kDa). I'm sorry if this information may not have been made explicit on the datasheet. Unfortunately I have no knowledge of any of the antibodies being produced by the group mentioned. You have not clarified the application or type of sample intended with the antibody but ab73885 would be a good choice if intending to perform Western blotting or ELISA with human CUTL1. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Oct 18 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

軽鎖の種類

研究分野

関連製品

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (24)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Protein CASP antibody [2A10]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Protein CASP antibody [2A10]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Protein CASP antibody [2A10]

Immunohistochemistry free floating abreview for Anti-Protein CASP antibody [2A10]

Immunohistochemistry (Frozen sections) abreview for Anti-Protein CASP antibody [2A10]

Immunocytochemistry abreview for Anti-Protein CASP antibody [2A10]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Protein CASP antibody [2A10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Protein CASP antibody [2A10]

Western blot abreview for Anti-Protein CASP antibody [2A10]