Anti-Proteasome subunit beta type-7 抗体 (ab154745)
Key features and details
- Rabbit polyclonal to Proteasome subunit beta type-7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Proteasome subunit beta type-7 antibody -
製品の詳細
Rabbit polyclonal to Proteasome subunit beta type-7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig
-
免疫原
Recombinant fragment corresponding to Human Proteasome subunit beta type-7 aa 50-300.
Database link: Q99436 -
ポジティブ･コントロール
- A549, HeLa, HepG, HCT116 and mouse brain lysates; HeLa cells; Human MDA-MB157 xenograft.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154745の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity. -
組織特異性
Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues. -
配列類似性
Belongs to the peptidase T1B family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 511207 Cow
- Entrez Gene: 5695 Human
- Entrez Gene: 19177 Mouse
- Entrez Gene: 100037992 Pig
- Entrez Gene: 85492 Rat
- Omim: 604030 Human
- SwissProt: Q2TBP0 Cow
- SwissProt: Q99436 Human
see all -
別名
- Macropain chain Z antibody
- Multicatalytic endopeptidase complex chain Z antibody
- Proteasome (prosome macropain) subunit beta type 7 antibody
see all
画像
-
Western blot - Anti-Proteasome subunit beta type-7 antibody (ab154745)Anti-Proteasome subunit beta type-7 antibody (ab154745) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 30 kDa
12% SDS PAGE -
Western blot - Anti-Proteasome subunit beta type-7 antibody (ab154745)Anti-Proteasome subunit beta type-7 antibody (ab154745) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg
Predicted band size: 30 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit beta type-7 antibody (ab154745)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Proteasome subunit beta type-7 with ab154745 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type-7 antibody (ab154745)Immunohistochemical analysis of paraffin-embedded MDA-MB-157 xenograft labeling Proteasome subunit beta type-7 with ab154745 at 1/100 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab154745 は 1 報の論文で使用されています。
- Auvin S et al. A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cells. Life Sci Alliance 2:N/A (2019). PubMed: 31431473