Anti-Proteasome Activator Subunit 4/PSME4 抗体 (ab5620)
Key features and details
- Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Proteasome Activator Subunit 4/PSME4 antibody
Proteasome Activator Subunit 4/PSME4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Zebrafish
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免疫原
Synthetic peptide corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 1620-1634.
Sequence:TKLPKKRKRDPGSVG
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ポジティブ･コントロール
- WB: Hela, Jurkat whole cell lysate, Mouse testis tissue lysate. ICC: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5620の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/600.
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| WB |
1/250 - 1/5000. Detects a band of approximately 200 kDa.
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| 特記事項 |
|---|
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ICC/IF
1/600. |
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WB
1/250 - 1/5000. Detects a band of approximately 200 kDa. |
ターゲット情報
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機能
Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair. -
配列類似性
Contains 6 HEAT repeats. -
細胞内局在
Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2). - Information by UniProt
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参照データベース
- Entrez Gene: 23198 Human
- Entrez Gene: 103554 Mouse
- Omim: 607705 Human
- SwissProt: Q14997 Human
- SwissProt: Q5SSW2 Mouse
- Unigene: 413801 Human
- Unigene: 481953 Mouse
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別名
- KIAA0077 antibody
- PA200 antibody
- Proteasome Activator 200 kDa antibody
see all
画像
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Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/250 dilution
Lane 1 : HeLa whole cell extract
Lane 2 : Jurkat whole cell extract
Lane 3 : Mouse testis tissue extract
Lane 4 : Mouse pancreas tissue extract
Lane 5 : Rat pancreas tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilutionA 200 kDa band corresponding to PSME4 was observed in HeLa, Jurkat, and mouse testis. In addition, a 140 kDa band corresponding to an isoform of PSME4 was observed across the cell lines and tissues tested.
Blocking - 5% skimmed milk
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Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg
Secondary
HRP-conjugated anti-rabbit
Developed using the ECL technique.
Observed band size: 160,200 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab5620 は 4 報の論文で使用されています。
- Johnson KO et al. Postsynaptic NMDA Receptor Expression Is Required for Visual Corticocollicular Projection Refinement in the Mouse Superior Colliculus. J Neurosci 43:1310-1320 (2023). PubMed: 36717228
- Pickering AM & Davies KJ Differential roles of proteasome and immunoproteasome regulators Pa28aß, Pa28? and Pa200 in the degradation of oxidized proteins. Arch Biochem Biophys 523:181-90 (2012). WB ; Mouse . PubMed: 22564544
- Greenhaff PL et al. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol Metab 295:E595-604 (2008). WB ; Human . PubMed: 18577697
- Zhang L et al. Effects of aging and dietary restriction on ubiquitination, sumoylation, and the proteasome in the spleen. FEBS Lett 581:5543-7 (2007). PubMed: 17991438