Anti-Proteasome 26S S3/PSMD3 抗体 (ab154963)
Key features and details
- Rabbit polyclonal to Proteasome 26S S3/PSMD3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Proteasome 26S S3/PSMD3 antibody
Proteasome 26S S3/PSMD3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Proteasome 26S S3/PSMD3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
-
免疫原
Recombinant fragment corresponding to Human Proteasome 26S S3/PSMD3 aa 1-240.
Database link: O43242 -
ポジティブ･コントロール
- A549, Hela, Jurkat, NT2D1, NIH 3T3 whole cell lysates; Cal27 xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154963の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ターゲット情報
-
機能
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
配列類似性
Belongs to the proteasome subunit S3 family.
Contains 1 PCI domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 5709 Human
- Entrez Gene: 22123 Mouse
- Entrez Gene: 287670 Rat
- SwissProt: O43242 Human
- SwissProt: P14685 Mouse
- Unigene: 12970 Human
- Unigene: 12194 Mouse
-
別名
- 26S proteasome non ATPase regulatory subunit 3 antibody
- 26S proteasome non-ATPase regulatory subunit 3 antibody
- 26S proteasome regulatory subunit RPN3 antibody
see all
画像
-
Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (ab154963)Anti-Proteasome 26S S3/PSMD3 antibody (ab154963) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg/ml
Predicted band size: 61 kDa
7.5% SDS PAGE -
Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (ab154963)Anti-Proteasome 26S S3/PSMD3 antibody (ab154963) at 1/500 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 26S S3/PSMD3 antibody (ab154963)Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling Proteasome 26S S3/PSMD3 with ab154963 at 1/500.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab154963 は論文での使用が確認できていません。