AB26078

Anti-Proteasome 26S S2/PSMD2 抗体

Name: Anti-Proteasome 26S S2/PSMD2 抗体 (ab26078) | Abcam
Brand: Abcam Limited
SKU: AB26078
Price: 76000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Proteasome 26S S2/PSMD2 antibody

(1 Review)

(2 Publications)

Goat Polyclonal Proteasome 26S S2/PSMD2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PSMD2 aa 850 to C-terminus.

別名を表示する

TRAP2, PSMD2, 26S proteasome non-ATPase regulatory subunit 2, 26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit S2, 26S proteasome subunit p97, Protein 55.11, Tumor necrosis factor type 1 receptor-associated protein 2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (AB26078)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (AB26078)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 μg/mL followed by Alexa Fluor 488 secondary antibody (2μg/mL). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. The nuclei were stained with DAPI (blue). Negative control : Unimmunized goat IgG (10μg/mL) followed by Alexa Fluor 488 secondary antibody (2μg/mL).

Cytoplasmic and some nuclear staining is observed.

Flow Cytometry (Intracellular) - Anti-Proteasome 26S S2/PSMD2 antibody (AB26078)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Proteasome 26S S2/PSMD2 antibody (AB26078)

Flow cytometry analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 μg/mL (blue) followed by Alexa Fluor 488 secondary antibody (1μg/mL) (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton.

IgG control : Unimmunized goat IgG (black) followed by Alexa Fluor 488 secondary antibody.

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human PSMD2 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13200

特異性

ab26078 recognises Proteasome 26S, subunit S2 .

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 26S S2 also known as PSMD2 is a subunit of the 26S proteasome. It plays an important role in the degradation of ubiquitinated proteins a process essential for maintaining protein homeostasis. The molecular mass of Proteasome 26S S2 is around 102 kDa. It is expressed in various tissues including the brain heart and muscle. The protein facilitates the recognition and unfolding of substrates destined for proteolysis by the 26S proteasome an activity that is instrumental in controlling the quality and abundance of proteins within the cell.

Biological function summary

Proteasome 26S S2 is an integral part of the 26S proteasome complex a large protease complex with a 20S core particle and 19S regulatory particles. This complex is responsible for degrading most intracellular proteins and is vital for regulating numerous cellular processes such as cell cycle control DNA repair and signal transduction. PSMD2 is linked with the assembly and stabilization of the 19S regulatory particle influencing the efficiency of the proteasome in recognizing and processing polyubiquitinated proteins.

Pathways

Proteasome 26S S2 is involved in ubiquitin-proteasome pathway and cell cycle regulation. In the ubiquitin-proteasome pathway it works with ubiquitin ligases and deubiquitinating enzymes to ensure selective and timely protein turnover. It is also associated with important regulatory proteins like CDC48 VCP/P97 which are involved in the ATP-dependent unfolding of proteins before proteasomal degradation. These pathways are essential for maintaining cellular protein homeostasis and managing cell cycle transitions suggesting an important regulatory role played by Proteasome 26S S2.

Proteasome 26S S2 is linked to neurodegenerative diseases and cancers. In neurodegenerative diseases such as Parkinson's disease dysfunction of the proteasome can lead to the accumulation of misfolded proteins thereby exacerbating disease progression. In cancers overexpression of Proteasome 26S S2 and other proteasomal subunits could promote the degradation of tumor suppressor proteins fueling carcinogenesis. It connects with proteins like PINK1 and PARKIN in neurodegeneration and interacts with p53 in cancer pathways indicating its critical involvement in disease mechanisms.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.. Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.

See full target information PSMD2

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental biology and medicine (Maywood, N.J.) 248:217-231 PubMed36740764

2023

Weighted gene co-expression network analysis reveals the hub genes associated with pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Shengyan Wang,Dejun Sun,Chuanchuan Liu,Yong Guo,Jie Ma,Ri-Li Ge,Sen Cui

Proceedings of the National Academy of Sciences of 117:19425-19434 PubMed32719113

2020

Krüppel-like factor 17 upregulates uterine corin expression and promotes spiral artery remodeling in pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Can Wang,Zhiting Wang,Meiling He,Tiantian Zhou,Yayan Niu,Shengxuan Sun,Hui Li,Ce Zhang,Shengnan Zhang,Meng Liu,Ying Xu,Ningzheng Dong,Qingyu Wu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 26S S2/PSMD2 抗体

Anti-Proteasome 26S S2/PSMD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (AB26078)

Flow Cytometry (Intracellular) - Anti-Proteasome 26S S2/PSMD2 antibody (AB26078)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise