Anti-Proteasome 20S LMP7 抗体 (ab3329)

Thank you for sending me this image. This data will be used to keep records of how our products are performing and for internal invetigation if needed. I noted that all tissue lysates that you have used have given the same non-specific signal. However I was wondering if you had tried a positive control lysate with your experiments. I ask this because according to SwissProt, in mouse tissue,this protreasome is expressed in spleen, thymus, lung, liver, heart and, at a very low level, in kidney.However it is not expressed in brain nor testis. My concern is that whilethe antibody may be recognizing non-specifically it may still be performing as there is not expression in the brain. This information is in the general annotations on this page: http://www.uniprot.org/uniprot/P28063 I would highly recommend using a control tissue lysate such as ab7937 Spleen (Mouse) Whole Cell Lysate - normal tissue ( https://www.abcam.com/Spleen-Mouse-Whole-Cell-Lysate-normal-tissue-ab7937.html). If you have any questions please do not hesitate to contact me.

Thank you for taking time to send us your protocols. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab38543. Spotty/sandy looking blots are often the result of aggregates in the block or antibody. I would recommend when preparing the milk in TBST that you stir it with a stir bar for quite some time. This will help dissolve particulate matter that can stick to your blot. Filtering the milk will also remove any milk that has not dissolved. I would also suggest that you centrifuge the antibody before use and use the supernatant from the top should aggregates appear. You may use the supernatant at recommended dilutions without a reduction in quality. I would also recommend using a lower concentration for your secondary antibody. Some people have noted that rabbit secondaries used at high concentration may cause issues like this. To increase target signal, I would suggest using a higher concentration of ant- SMURF 2 antibody. This product has been used successfully at concentrations as high as 1:50. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. In regards to ab3329. I've seen that others have posted abreviews which have shown a higher non-specific band at about 66 kDa. Is this about the range of the higher band that you have reported? Do you have an image that you might send us? Would it also be possible to send me the original order number or purchase order for these products. If we need to replace them having that information will expedite the process. I hope this information is helpful, and I thank you for your cooperation.

Thank you for contacting us. I would like to ask you if you might send me the protocols used with these productsso that we can gather further information regarding the samples tested and the protocol used. Once we receive your response, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. For our records could you please include the following details in your protocols for each antibody? What tissue type was used? How was the tissue prepared, including lysis buffer and conditions? Were the samples denatured? How much total protein was loaded per well? What were the running and transfer conditions and buffers? Did you use a loading control? Did you usethe recommended positive controls? What were the results of those controls? What blocking reagents did you use? You have mentioned that milk and BSA were used for ab3329, what were the concentrations of these in your block? What incubation times and temperatures did you use? What concentrations did you use the primary antibody concentration at? What incubation times, temperatures and reagents did you use? What was the secondary antibody? What were the concentrations, incubation times, temperatures and reagents used with this? Were different conditions tried, including different antibody concentrations and incubation times? What wash steps did you use? Which detection system did you use? I look forward to hearing form you soon.