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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Proteasome 20S LMP2 抗体 [EPR13785] (ab184172)

Submit a review Submit a question References (7)
Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
  • Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
  • Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-Proteasome 20S LMP2 antibody [EPR13785]
    Proteasome 20S LMP2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Ramos, A431 and Raji cell lysates; HL60 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13785

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184172の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/100000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
ICC/IF
1/100.
IP
1/50.
特記事項
WB
1/10000 - 1/100000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
ICC/IF
1/100.
IP
1/50.

ターゲット情報

  • 機能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.

  • 配列類似性

    Belongs to the peptidase T1B family.

  • 発生段階

    Highly expressed in immature dendritic cells (at protein level).

  • 翻訳後修飾

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Beta1i antibody
    • Large multifunctional peptidase 2 antibody
    • Large multifunctional protease 2 antibody
    • LMP 2 antibody
    • LMP2 antibody
    • Low molecular mass protein 2 antibody
    • Macropain chain 7 antibody
    • MGC70470 antibody
    • Multicatalytic endopeptidase complex chain 7 antibody
    • OTTHUMP00000062982 antibody
    • Proteasome (prosome macropain) subunit beta type 9 antibody
    • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) antibody
    • Proteasome beta 9 subunit antibody
    • Proteasome catalytic subunit 1i antibody
    • Proteasome chain 7 antibody
    • Proteasome related gene 2 antibody
    • Proteasome subunit beta 6i antibody
    • Proteasome subunit beta type 9 antibody
    • Proteasome subunit beta type-9 antibody
    • Proteasome subunit beta-1i antibody
    • PSB9_HUMAN antibody
    • PSMB 9 antibody
    • PSMB6i antibody
    • PSMB9 antibody
    • Really interesting new gene 12 protein antibody
    • RING 12 antibody
    • RING12 antibody
    • RING12 protein antibody
    see all

画像

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
    Lanes 1 - 2: Merged signal (red and green). Green - ab184172 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab184172 was shown to specifically react with Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab184172 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HL-60 (Human promyelocytic leukemia cell line) cells labeling Proteasome 20S LMP2 using ab184172 at 1/100 dilution (red). A Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    All lanes : Anti-EXTL1 antibody - C-terminal (ab174172) at 1/100000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

    Immunoprecipitation analysis of Raji cells labeling Proteasome 20S LMP2 using ab184172 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Raji lysates. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

参考文献 (7)

ab184172 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184172 は 7 報の論文で使用されています。

  • Zhan L  et al. LC3 and NLRC5 interaction inhibits NLRC5-mediated MHC class I antigen presentation pathway in endometrial cancer. Cancer Lett 529:37-52 (2022). PubMed: 34974132
  • Ng S  et al. STUB1 is an intracellular checkpoint for interferon gamma sensing. Sci Rep 12:14087 (2022). PubMed: 35982220
  • Tripodi L  et al. Immunoproteasome Inhibition Ameliorates Aged Dystrophic Mouse Muscle Environment. Int J Mol Sci 23:N/A (2022). PubMed: 36498987
  • Farini A  et al. Defective dystrophic thymus determines degenerative changes in skeletal muscle. Nat Commun 12:2099 (2021). PubMed: 33833239
  • Farini A  et al. PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy. Front Physiol 11:403 (2020). PubMed: 32508664
  • Smith BC  et al. The impact of regional astrocyte interferon-? signaling during chronic autoimmunity: a novel role for the immunoproteasome. J Neuroinflammation 17:184 (2020). PubMed: 32532298
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371

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