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リコンビナントRabMAb

Anti-Proteasome 20S beta 6 抗体 [EPR9684] (ab150392)

Reviews (3) Submit a question References (2)
Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
  • Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9684] to Proteasome 20S beta 6
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Proteasome 20S beta 6 antibody [EPR9684]
    Proteasome 20S beta 6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9684] to Proteasome 20S beta 6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Recombinant Human Proteasome 20S beta 6 protein (ab116187) can be used as a positive control in WB. Human fetal liver, NCI-H460 and Caco 2 cell lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9684

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150392の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.

  • 配列類似性

    Belongs to the peptidase T1B family.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DELTA antibody
    • LMPY antibody
    • Macropain delta chain antibody
    • MGC5169 antibody
    • Multicatalytic endopeptidase complex delta chain antibody
    • Proteasome (prosome macropain) subunit beta type 6 antibody
    • Proteasome beta 6 subunit antibody
    • Proteasome catalytic subunit 1 antibody
    • Proteasome delta chain antibody
    • Proteasome subunit beta 6 antibody
    • Proteasome subunit beta type 6 antibody
    • Proteasome subunit beta type-6 antibody
    • Proteasome subunit delta antibody
    • Proteasome subunit Y antibody
    • PSB6_HUMAN antibody
    • PSMB6 antibody
    • PSY large multifunctional protease Y antibody
    • Y antibody
    see all

画像

  • Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
    Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
    All lanes : Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392) at 1/1000 dilution

    Lane 1 : Human fetal liver cell lysate
    Lane 2 : NCI-H460 cell lysate
    Lane 3 : Caco 2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
    Immunofluorescent analysis of HeLa cells labelling Proteasome 20S beta 6 with ab150392 at 1/100 dilution.
  • Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
    Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)

参考文献 (2)

ab150392 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab150392 は 2 報の論文で使用されています。

  • Kim M  et al. Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction. Nat Commun 14:4092 (2023). PubMed: 37433777
  • Luan Y  et al. Chronic Caffeine Treatment Protects Against a-Synucleinopathy by Reestablishing Autophagy Activity in the Mouse Striatum. Front Neurosci 12:301 (2018). PubMed: 29770111

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Proteasome 20S beta 6 antibody [EPR9684]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 28 2019

Western blot abreview for Anti-Proteasome 20S beta 6 antibody [EPR9684]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
U2OS cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 03 2018

Western blot abreview for Anti-Proteasome 20S beta 6 antibody [EPR9684]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 26 2018

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