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リコンビナントRabMAb

Anti-Proteasome 20S alpha 5/PSMA5 抗体 [EPR5833] (ab109533)

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Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
  • Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5833] to Proteasome 20S alpha 5/PSMA5
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833]
    Proteasome 20S alpha 5/PSMA5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5833] to Proteasome 20S alpha 5/PSMA5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, Hela, 293T and JAR cell lysates; Human brain tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR5833

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109533の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

  • 組織特異性

    Expressed in fetal brain (at protein level).

  • 配列類似性

    Belongs to the peptidase T1A family.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Macropain zeta chain antibody
    • Multicatalytic endopeptidase complex zeta chain antibody
    • Proteasome (prosome macropain) subunit alpha type 5 antibody
    • Proteasome alpha5 subunit antibody
    • Proteasome component 5 antibody
    • Proteasome subunit alpha type 5 antibody
    • Proteasome subunit alpha type-5 antibody
    • Proteasome subunit zeta antibody
    • Proteasome zeta chain antibody
    • PSA5_HUMAN antibody
    • PSC5 antibody
    • PSMA5 antibody
    • ZETA antibody
    see all

画像

  • Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)

    ab109533, at 1/100 dilution, staining Proteasome 20S alpha 5/PSMA5 in paraffin embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)

参考文献 (0)

ab109533 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109533 は論文での使用が確認できていません。

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