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リコンビナントRabMAb

Anti-Proteasome 20S alpha 2/HC3 抗体 [EPR5453] (ab109525)

Reviews (1)Q&A (1)References (4)
Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
  • Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]
    Proteasome 20S alpha 2/HC3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR5453

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109525の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

(Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF
1/50 - 1/100.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

(Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF
1/50 - 1/100.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.

  • 配列類似性

    Belongs to the peptidase T1A family.

  • 翻訳後修飾

    Phosphorylated on tyrosine residues; which may be important for nuclear import.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • HC3 antibody
    • Macropain subunit C3 antibody
    • MU antibody
    • Multicatalytic endopeptidase complex subunit C3 antibody
    • PMSA2 antibody
    • Proteasome (prosome macropain) subunit alpha type 2 antibody
    • Proteasome alpha 2 subunit antibody
    • Proteasome component C3 antibody
    • Proteasome subunit alpha type 2 antibody
    • Proteasome subunit alpha type-2 antibody
    • Proteasome subunit HC3 antibody
    • PSA2_HUMAN antibody
    • PSC2 antibody
    • PSC3 antibody
    • PSMA 2 antibody
    • psmA2 antibody
    see all

画像

  • Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
    Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
    All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)

    ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2/HC3 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).

    Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)

    ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2/HC3 in HeLa cells by Immunofluorescence.

  • Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
    Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)

参考文献 (4)

ab109525 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109525 は 4 報の論文で使用されています。

  • Ni HM  et al. Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)-Mixed Lineage Kinase Domain-Like Protein (MLKL)-Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers. Am J Pathol N/A:N/A (2019). PubMed: 31026418
  • Alber AB  et al. Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates. Mol Cell 71:1079-1091.e9 (2018). PubMed: 30146318
  • Wang S  et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget 7:17681-98 (2016). PubMed: 26769846
  • Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . PubMed: 24556681

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Proteasome 20S alpha 2 antibody [EPR5453]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12 %)
Sample
Human Cell lysate - whole cell (HaCaT human immortalized keratinocyte cell line, 3)
Specification
HaCaT human immortalized keratinocyte cell line, 3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Iva Doleckova

Verified customer

投稿 May 31 2014

Question

What is the estimate of non-IgG protein in this antibody preparation? 

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Answer

Thank you for your inquiry. Regarding ab109525, we have not actually measured non-IgG protein concentration. The only known non-IgG protein in our antibody is BSA (0.05%). Please contact us for the specific IgG concentration of the lot you received.    I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

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