Anti-Proteasome 20S alpha 1+2+3+5+6+7 抗体 [MCP231] (ab22674)
![Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)](https://www.abcam.co.jp/ps/products/22/ab22674/Images/ab22674-32467-anti-proteasome-20s-alpha-123567-antibody-mcp231-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)")
Key features and details
- Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] -
製品の詳細
Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: a wide range of other species
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免疫原
Full length protein corresponding to Proteasome 20S alpha 1+2+3+5+6+7. Dinitrophenylated proteasomes
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エピトープ
Reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved. -
ポジティブ･コントロール
- Human placental proteasome.
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特記事項
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Dilute antibody to working dilution in PBS, pH 7.2 - 7.4. Store at 4°C and use within 1 month. Store unopened vial at -20°C for long term storage. Avoid freeze/thaw cycles.
This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7. -
ポリ/モノ
モノクローナル -
クローン名
MCP231 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22674の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The proteasome is widely recognised as the central enzyme of non lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP dependent pathway of protein degradation. The catalytic core of this unusually large complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha subunits comprise the outer rings and the beta subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha7/beta7/beta7/alpha7) with a member of each sub family represented in each particle. Each subunit is located in a unique position within the alpha or beta rings. 20S Proteasomes degrade only unfolded proteins in an energy independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 5682 Human
- Entrez Gene: 5683 Human
- Entrez Gene: 5684 Human
- Entrez Gene: 5686 Human
- Entrez Gene: 5687 Human
- Entrez Gene: 5688 Human
- Omim: 176842 Human
- Omim: 602854 Human
see all -
別名
- Proteasome component antibody
- Proteasome subunit alpha type 1 antibody
- Proteasome subunit alpha type 2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)
ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)](https://www.abcam.co.jp/ps/products/22/ab22674/Images/ab22674-32466-anti-proteasome-20s-alpha-123567-antibody-mcp231-flow-cytometry.jpg)
Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab22674 は 28 報の論文で使用されています。
- Koçberber Z et al. The role of proteasome activators PA28αβ and PA200 in brown adipocyte differentiation and function. Front Endocrinol (Lausanne) 14:1176733 (2023). PubMed: 37201100
- Kim M et al. Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction. Nat Commun 14:4092 (2023). PubMed: 37433777
- Wang F et al. SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage. Research (Wash D C) 6:0091 (2023). PubMed: 37223481
- Kammerl IE et al. Activation of immune cell proteasomes in peripheral blood of smokers and COPD patients: implications for therapy. Eur Respir J 59:N/A (2022). PubMed: 34561290
- Wong DCS et al. CRYPTOCHROMES promote daily protein homeostasis. EMBO J 41:e108883 (2022). PubMed: 34842284