Anti-Proteasome 20S alpha + beta 抗体 (ab22673)

Thank you for contacting us yesterday.

I am sorry but as you are aware we do not currently have any of the Anti-Proteasome 20S alpha + beta antibody (ab22673). As discussed over the phone, I have had a look for antibodies which may serve as suitable alternatives for you. Please have a look at the following antibodies to see if they may be suitable for your experiments:

Rabbit polyclonal to Proteasome 20S LMP7 ab3329

Rabbit polyclonal to Proteasome 20S C2 ab3325

Rabbit polyclonal to Proteasome 20S LMP2 ab42987

Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7 ab22674

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Thank you for your reply.

I can change the antibody on your current order from ab22673 for ab109525 if you would like. As this is in stock, if you let me know you would like for me to do this, it can be with you by tomorrow.

The antibody has been used both in WB and IF and should therefore be suitable for your experiments. Please be aware that this antibody is raised against the Proteasome 20S alpha 2 and will detect this protein. It has been shown to work with mouse, rat and human samples.

I hope this information has been of help. If you would prefer to reply in Spanish again please do, our Spanish speaking Scientific Support specialist returned to the office yesterday and would be happy to help.

I look forward to receiving your reply.

Thank you for contacting us and your interest in our products.

Unfortunately, we do not have a direct alternative for the Anti-Proteasome 20S alpha + beta antibody (ab22673). This antibody detects a combination of alpha and beta proteasome subunits. The only other antibodies we have are directed against the alpha subunit (such as mouse monoclonal https://www.abcam.com/Proteasome-20S-alpha-1-2-3-5-6-7-antibody-MCP231-ab22674.htmlor rabbit monoclonal https://www.abcam.com/Proteasome-20S-alpha-2-antibody-EPR5453-ab109525.html) or beta subunit (rabbit polyclonal https://www.abcam.com/Proteasome-20S-beta-6-antibody-C-terminal-ab135537.htmlor mouse monoclonal https://www.abcam.com/Proteasome-20S-beta-6-antibody-ab58081.html).

If you would like to describe in a bit more detail what part of the proteasome you are hoping to detect, I can try to help choose an appropriate alternative. Otherwise, all our antibodies directed towards Proteasome 20S can be found from the following link:

https://www.abcam.com/index.html?pageconfig=searchresults&search=Proteasome 20S&pt=1&sd=2&fViewMore=1

I hope this information has been of some help. If you have any further questions, please do not hesitate to ask.

Thank you for your reply.

The credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

Please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

Let me know if you have any questions or ifthere is anything else that we can do for you, and I'll be happy to help you further.

Thank you for your reply and for sending the additional information.

I would be happyto issue a credit note for the customer. Do you have the order number or PO number that this was ordered on? I found one going to Interlab from February 7, order # ***. Is this the correct order?

I look forward to hearing from you and resolving this promptly. Have a great day!

Thank you for contacting us and forwarding the customer's protocol details.

I do have a few additional questions, so that I better understand the situation.

The secondary is listed as HRP conjugated, and the detection system is listed as ECL, however the image shows fluorescent deteciton.Is the proteasome stainingin the green or red channel? What other protein has been stained, and what kind of antibody was used?

I've looked through some literature about the expression of this protein, and the subunits can be found in the nucleus or cytoplasm. What kind of staining does the customer expect to see in these hippocampal and cortical neurons?

The customer's protocol looks good, but to reduce background I would recommend blocking the cells with 10% serum from the host of the secondary antibody (10% goat serum in this case) which often improves the results. We do fully guarantee this antibody to work in rat ICC, so if the customer would prefer, I'd be happy to send a replacement or issue a credit or refund.

I look forward to hearing from you. Please let me know if the customer has any further questions, or if there is anything else that we can do for you.