Anti-Proteasome 19S S5A/ASF 抗体 (ab154935)
Key features and details
- Rabbit polyclonal to Proteasome 19S S5A/ASF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Proteasome 19S S5A/ASF antibody
Proteasome 19S S5A/ASF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Proteasome 19S S5A/ASF -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
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免疫原
Recombinant fragment corresponding to Human Proteasome 19S S5A/ASF aa 80-319.
Database link: P55036 -
ポジティブ･コントロール
- A549, HeLa, HepG2 and HCT116 whole cell lysates; A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. -
配列類似性
Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain. -
ドメイン
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 5710 Human
- Entrez Gene: 19185 Mouse
- Omim: 601648 Human
- SwissProt: P55036 Human
- SwissProt: O35226 Mouse
- Unigene: 505059 Human
- Unigene: 2261 Mouse
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別名
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
see all
画像
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Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab154935)Anti-Proteasome 19S S5A/ASF antibody (ab154935) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S5A/ASF antibody (ab154935)Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling Proteasome 19S S5A/ASF with ab154935 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154935 は論文での使用が確認できていません。